Label-free electrochemical immunoassay for the detection of human chorionic gonadotropin hormone

被引:79
作者
Kerman, Kagan [1 ]
Nagatani, Naoki
Chikae, Miyuki
Yuhi, Teruko
Takamura, Yuzuru
Tamiya, Eiichi
机构
[1] JAIST, Sch Mat Sci, Nomi City, Ishikawa 9231292, Japan
[2] Japan Sci & Technol Agcy, Nomi City, Ishikawa 9231211, Japan
关键词
D O I
10.1021/ac051762l
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Here we report on a new and rapid immunoassay for the label-free voltammetric detection of human chorionic gonadotropin hormone (hCG) in urine. Monitoring the changes in the current signals of antibodies (Abs) before and after the binding of the antigen (Ag) provides the basis for an immunoassay that is simple, rapid, and cost-effective. Since hCG is found at highly elevated levels in pregnant female urine with the range of 30 000-200 000 mIU/mL (similar to 30- 200 nM) by 8-10 weeks into pregnancy, its label-free electrochemical detection was achieved by using our method. The coverage of the electrode surface with the Ab and the incubation time with the target Ag were optimized for the detection of hCG. The limit of detection of our method was calculated to be 15 pM (n = 3, similar to 15 mIU/mL) in synthetic hCG samples and 20 pM (n = 3, similar to 20 mIU/mL) in human urine. The electrochemical results for the detection of hCG in the urine samples were in agreement with the results obtained using a reference system, enzyme-linked immunosorbent assay. Further research about the intrinsic electroactivity of Abs and their target molecules would surely provide new and sensitive screening assays, as well as extensive data regarding their interaction mechanisms.
引用
收藏
页码:5612 / 5616
页数:5
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