Sandwich-type electrochemiluminescence immunosensor based on N-(aminobutyl)-N-ethylisoluminol labeling and gold nanoparticle amplification

被引：61

作者：

Tian, Dayong ^{[1
]}

Duan, Chunfeng ^{[1
]}

Wang, Wei ^{[1
]}

Li, Na ^{[1
]}

Zhang, Hao ^{[1
]}

Cui, Hua ^{[1
]}

Lu, Yingyu ^{[2
]}

机构：

[1] Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China

[2] AnHui Med Univ, Affiliated Hosp 1, Hefei 230026, Anhui, Peoples R China

来源：

TALANTA | 2009年 / 78卷 / 02期

关键词：

Electrochemluminescence immunoassay; Human immunoglobulin G; N-(aminobutyl)-N-ethylisoluminol; Gold nanopaticles; C-REACTIVE PROTEIN; ELECTROGENERATED CHEMILUMINESCENCE; DNA HYBRIDIZATION; ELECTRODE; IMMUNOASSAY; INHIBITION; BIOSENSOR; LUMINOL; SYSTEM; ABEI;

D O I：

10.1016/j.talanta.2008.11.037

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel electrochemiluminescence (ECL) sandwich-type immunosensor for human immunoglobulin G (hIgG) on a gold nanoparticle modified electrode was developed by using W(aminobutyl)-N-ethylisoluminol (ABEI) labeling. The primary antibody, goat-anti-human IgG was first immobilized on a gold nanoparticle modified electrode, then the antigen (human IgG) and the ABEI-labeled second antibody was conjugated successively to form a sandwich-type immunocomplex. ECL was carried out with a double-step potential in carbonate buffer solution (CBS) containing 1.5 mM H2O2. The ECL intensity increased linearly with the concentration of hlgG over the range 5.0-100 ng/mL. The limit of detection was 1.68 ng/mL(S/N=3). The relative standard deviation was 3.79% at 60 ng/mL(n=9). The present immunosensor is simple and sensitive. It has been successfully applied to the detection of hlgG in human serums. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：399 / 404

页数：6

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