Selective usage of VH genes in adult human B lymphocyte repertoires

被引:22
作者
Davidkova, G
Pettersson, S
Holmberg, D
Lundkvist, I
机构
[1] HUDDINGE UNIV HOSP,KAROLINSKA INST,DIV CLIN IMMUNOL,S-14186 HUDDINGE,SWEDEN
[2] KAROLINSKA INST,NOVUM,CTR BIOTECHNOL,HUDDINGE,SWEDEN
[3] UMEA UNIV,DEPT MOL & CELL BIOL,UMEA,SWEDEN
关键词
D O I
10.1046/j.1365-3083.1997.d01-376.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The authors have compared the VH gene utilization patterns among small resting immunocompetent B cells and large naturally activated B lymphocytes of healthy human adults. They employed a non-radioactive RNA in situ hybridization technique that allows detection of VH gene family expression at the single cell level. Pokeweed mitogen stimulated and unmanipulated mononuclear cells from peripheral blood and spleen of unrelated individuals were hybridized to digoxigenin-labelled antisense RNA probes specific for human VH families 1-6 and for the constant region genes C mu and C gamma. The observed VH gene family utilization patterns did not correlate with the genomic complexity of human VH genes. The VH3 gene family was most frequently used among resting B cells in both peripheral blood and spleen. Among naturally activated lymphocytes the VH6 gene was markedly over-represented, while expression of the VH1 and VH3 gene families was decreased. The data show that V-region mediated selection participates in shaping the peripheral antibody repertoire in healthy adults.
引用
收藏
页码:62 / 73
页数:12
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