Lucky Imaging: Improved Localization Accuracy for Single Molecule Imaging

被引：11

作者：

Cronin, Brid ^{[1
]}

de Wet, Ben ^{[2
]}

Wallace, Mark I. ^{[1
]}

机构：

[1] Univ Oxford, Chem Res Lab, Oxford, England

[2] Univ Oxford, Sir William Dunn Sch Pathol, Oxford, England

来源：

BIOPHYSICAL JOURNAL | 2009年 / 96卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

OPTICAL RECONSTRUCTION MICROSCOPY; FLUORESCENCE MICROSCOPY; HIGH-RESOLUTION; T-CELLS; ACTIVATION; DIFFUSION; NANOSCOPY; DYNAMICS; PROBES; LIMIT;

D O I：

10.1016/j.bpj.2008.12.3945

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

We apply the astronomical data-analysis technique, Lucky imaging, to improve resolution in single molecule fluorescence microscopy. We show that by selectively discarding data points from individual single-molecule trajectories, imaging resolution can be improved by a factor of 1.6 for individual fluorophores and up to 5.6 for more complex images. The method is illustrated using images of fluorescent dye molecules and quantum dots, and the in vivo imaging of fluorescently labeled linker for activation of T cells.

引用

收藏

页码：2912 / 2917

页数：6

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