Chemiluminometric method for the determination of glycerol in wine by flow-injection analysis with co-immobilized glycerol dehydrogenase NADH oxidase

被引：20

作者：

Kiba, N

Azuma, N

Furusawa, M

机构：

[1] Dept. of Appl. Chem. and Biotech., Faculty of Engineering, Yamanashi University

来源：

TALANTA | 1996年 / 43卷 / 10期

关键词：

chemiluminescence; enzyme reactor; flow injection; glycerol; wine;

D O I：

10.1016/0039-9140(96)01969-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection method for the determination of glycerol in wine is described. Glycerol dehydrogenase and NADH oxidase were co-immobilized on poly (vinyl alcohol) beads and incorporated in a flow-injection system. The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate (III) reaction. Wine was diluted 1000-fold with water and sample solution (50 mu l) was injected into the carrier stream. The calibration graph was linear in the range 3 x 10(-7)-3 x 10(-4) M; the detection limit was 7 x 10(-8) M and the sample throughout was 30 h(-1) without carryover.

引用

页码：1761 / 1766

页数：6

共 14 条

[1] SULFITE-BINDING POWER OF WINES AND CIDERS .1. EQUILIBRIUM CONSTANTS FOR DISSOCIATION OF CARBONYL BISULFITE COMPOUNDS [J].