Marker Constituents of the Natural Antioxidant Eucalyptus Leaf Extract for the Evaluation of Food Additives

被引：70

作者：

Amakura, Yoshiaki ^{[1
]}

Yoshimura, Morio ^{[1
]}

Sugimoto, Naoki ^{[2
]}

Yamazaki, Takeshi ^{[2
]}

Yoshida, Takashi ^{[1
]}

机构：

[1] Matsuyama Univ, Coll Pharmaceut Sci, Matsuyama, Ehime 7908578, Japan

[2] Natl Inst Hlth Sci, Div Foods Addit, Setagaya Ku, Tokyo 1588501, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 05期

关键词：

natural antioxidant; food additive; eucalyptus; Eucalyptus globulus; polyphenol; GRANULATION-INHIBITING PRINCIPLES; DIMERIC HYDROLYZABLE TANNIN; GLOBULUS LABILL; ACID-DERIVATIVES; LEAVES; POLYPHENOLS; GLYCOSIDES;

D O I：

10.1271/bbb.80832

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to establish the marker constituents of the natural antioxidant food-additive Eucalyptus leaf extract, the UV-absorbing constituents of two eucalyptus leaf extracts registered as food additives (eucalyptus A and B) were investigated. Several major peaks on the reversed-phase HPLC chromatogram of eucalyptus A were characterized as gallic acid, ellagic acid, 3-O-beta-D-glucuronides of quercetin and kaempferol, and a hydrolyzable tannin dimer, oenothein B, by direct comparison with authentic specimens isolated from Eucalyptus globulus leaves. A new gallotannin was found in the E. globulus leaf extract, and its structure was found to be 1,2,3,6-tetra-O-galloyl-beta-D-galactose. Two major peaks on the HPLC chromatogram of eucalyptus B were identified as gallic acid and ellagic acid, indicative of degradation products from hydrolyzable tannins in the leaves. Considering the evaluation of antioxidant activity by radical scavenging ability, a standardization of eucalyptus leaf extract, including the antioxidative polyphenol, oenothein B, is proposed.

引用

页码：1060 / 1065

页数：6

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