The Proteome of Seed Development in the Model Legume Lotus japonicus

被引:67
作者
Dam, Svend [1 ,3 ]
Laursen, Brian S. [1 ,3 ]
Ornfelt, Jane H. [1 ,3 ]
Jochimsen, Bjarne [1 ,3 ]
Staerfeldt, Hans Henrik [4 ]
Friis, Carsten [4 ]
Nielsen, Kasper [4 ]
Goffard, Nicolas [5 ,6 ]
Besenbacher, Soren [7 ]
Krusell, Lene [1 ,3 ]
Sato, Shusei [8 ]
Tabata, Satoshi [8 ]
Thogersen, Ida B. [2 ,3 ]
Enghild, Jan J. [2 ,3 ]
Stougaard, Jens [1 ,3 ]
机构
[1] Univ Aarhus, Ctr Carbohydrate Recognit & Signalling, DK-8000 Aarhus, Denmark
[2] Univ Aarhus, Ctr Insoluble Prot Struct, DK-8000 Aarhus, Denmark
[3] Univ Aarhus, Dept Mol Biol, DK-8000 Aarhus, Denmark
[4] Tech Univ Denmark, Ctr Biol Sequence Anal, DK-2800 Lyngby, Denmark
[5] Inst Louis Malarde, F-98713 Papeete, France
[6] Australian Natl Univ, Res Sch Biol Sci, Genom Interact Grp, Australian Res Council Ctr Excellence Integrat Le, Canberra, ACT 2601, Australia
[7] Univ Aarhus, Bioinformat Res Ctr, DK-8000 Aarhus, Denmark
[8] Kazusa DNA Res Inst, Chiba 2920818, Japan
基金
新加坡国家研究基金会;
关键词
EMBRYOGENESIS ABUNDANT PROTEIN; STORAGE PROTEIN; GLUTAMINE-SYNTHETASE; MEDICAGO-TRUNCATULA; SOYBEAN OILS; STARCH; GENES; IDENTIFICATION; EXPRESSION; GLYCININ;
D O I
10.1104/pp.108.133405
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean (Glycine max) and pea (Pisum sativum), Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm, and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined, and this indicates that the composition of mature Lotus seed is more similar to soybean than to pea. In a first attempt to determine the seed proteome, both a two-dimensional polyacrylamide gel electrophoresis approach and a gel-based liquid chromatography-mass spectrometry approach were used. Globulins were analyzed by two-dimensional polyacrylamide gel electrophoresis, and five legumins, LLP1 to LLP5, and two convicilins, LCP1 and LCP2, were identified by matrix-assisted laser desorption ionization quadrupole/time-of-flight mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a gel-based liquid chromatography-mass spectrometry approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases, respectively. All of the proteome data, including the experimental data and mass spectrometry spectra peaks, were collected in a database that is available to the scientific community via a Web interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry, and regulation of seed development in Lotus. Together with a new Web interface (http://bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family.
引用
收藏
页码:1325 / 1340
页数:16
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