An ARIA-interacting AP2 domain protein is a novel component of ABA signaling

被引:45
作者
Lee, Sun-ji
Cho, Dong-im
Kang, Jung-youn
Kim, Soo Young [1 ]
机构
[1] Chonnam Natl Univ, Coll Agr & Life Sci, Dept Mol Biotechnol, Kwangju 500757, South Korea
关键词
Abscisic acid (ABA); ABF; abiotic stress; Arm protein; RESPONSIVE GENE-EXPRESSION; ARABIDOPSIS TRANSCRIPTION FACTOR; ABSCISIC-ACID; APETALA2-LIKE GENE; LEUCINE-ZIPPER; DROUGHT; COLD; AINTEGUMENTA; TRANSDUCTION; REGULATOR;
D O I
10.1007/s10059-009-0058-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
ADAP is an AP2-domain protein that interacts with ARIA, which, in turn, interacts with ABF2, a bZIP class transcription factor. ABF2 regulates various aspects of the abscisic acid (ABA) response by controlling the expression of a subset of ABA-responsive genes. Our expression analyses indicate that ADAP is expressed in roots, emerging young leaves, and flowers. We found that adap knockout mutant lines germinate more efficiently than wild-type plants and that the mutant seedlings grow faster. This suggests that ADAP is involved in the regulation of germination and seedling growth. Both germination and post-germination growth of the knockout mutants were partially insensitive to ABA, which indicates that ADAP is required for a full ABA response. The survival rates for mutants from which water was withheld were low compared with those for wild-type plants. The result shows that ADAP is necessary for the response to stress induced by water deprivation. Together, our data indicate that ADAP is a positive regulator of the ABA response and is also involved in regulating seedling growth. The role of ADAP is similar to that of ARIA, which is also a positive regulator of the ABA response. It appears that ADAP acts through the same ABA response pathway as ARIA.
引用
收藏
页码:409 / 416
页数:8
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