Human P2X7 pore function predicts allele linkage disequilibrium

被引:43
作者
Denlinger, Loren C.
Coursin, Douglas B.
Schell, Kathleen
Angelini, Giuditta
Green, Dawn N.
Guadarrama, Arturo G.
Halsey, Jennifer
Prabhu, Usha
Hogan, Kirk J.
Bertics, Paul J.
机构
[1] Univ Wisconsin, Sch Med & Publ Hlth, Sect Allergy Pulm & Crit Care Med, Dept Med, Madison, WI 53705 USA
[2] Univ Wisconsin, Sch Med & Publ Hlth, Dept Biomol Chem, Madison, WI 53705 USA
[3] Univ Wisconsin, Sch Med & Publ Hlth, Dept Anesthesiol, Madison, WI 53705 USA
[4] Univ Wisconsin, Sch Med & Publ Hlth, Ctr Comprehens Canc, Madison, WI 53705 USA
关键词
D O I
10.1373/clinchem.2005.065425
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Innate immune response amplification is achieved by leukocyte expression of the purinergic nucleotide receptor P2X(7), an extracellular nucleotide-gated pore. Previously, low P2X(7) pore activity in whole blood was associated with loss-of-function genotypes in correlation with a decreased ratio of lipopolysaccharide-stimulated tumor necrosis factor-a to interleukin-10, of relevance to a variety of infectious and inflammatory disorders. We hypothesized that evaluation of participants with discordance between the P2X(7) genotype and pore status would disclose additional alleles, linkage disequilibrium, and novel functional correlates of genotype to phenotype. Methods: Comparison of whole-blood pore results with restriction fragment length polymorphism data for known loss-of-function genotypes from 200 healthy participants optimized the diagnostic threshold for low pore activity by ROC curve analysis. We identified novel alleles and inferred haplotypes by sequencing outlier genomic templates and by linkage analysis. Results: With a refined threshold of low activity, a normal pore result had only a 2% probability of association with known loss-of-function variants. By contrast, the positive predictive value of low pore activity was 59% for identifying known alleles. DNA samples from this discordant group contained 28 P2X(7) sequence variations. Linkage analysis demonstrated that A1513C, T1729A, and G946A are inherited independently from one another, although these loss-of-function variants are in disequilibrium with other alleles. When we segregated pore activity data according to genotypes, nonsynonymous sequence variations (G474A and A1405G) appeared to exhibit modulatory effects on P2X7 pore activity. Conclusions: Direct analysis of pore activity demonstrates functional interactions between P2X7 alleles. The performance characteristics of the whole-blood pore assay enables correlation of genomic variation with concomitant investigation of functional performance in clinical studies. (c) 2006 American Association for Clinical Chemistry.
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收藏
页码:995 / 1004
页数:10
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