miR-15a inhibits cell proliferation and epithelial to mesenchymal transition in pancreatic ductal adenocarcinoma by down-regulating Bmi-1 expression

被引：111

作者：

Guo, Shixiang ^{[1
]}

Xu, Xuejun ^{[1
]}

Tang, Yichen ^{[1
]}

Zhang, Chaobin ^{[1
]}

Li, Jian ^{[1
]}

Ouyang, Yongsheng ^{[1
]}

Ju, Jingfang ^{[2
]}

Bie, Ping ^{[1
]}

Wang, Huaizhi ^{[1
]}

机构：

[1] Third Mil Med Univ, Inst Hepatopancreatobiliary Surg, Southwest Hosp, Chongqing 400038, Peoples R China

[2] SUNY Stony Brook, Dept Pathol, Translat Res Lab, Stony Brook, NY 11794 USA

来源：

CANCER LETTERS | 2014年 / 344卷 / 01期

基金：

中国国家自然科学基金;

关键词：

miR-15a; Bmi-1; EMT; Proliferation; Pancreatic ductal adenocarcinoma; CANCER; INVASION; SIGNATURE; PROFILES; METASTASIS; MICRORNAS; PROGNOSIS; DIAGNOSIS;

D O I：

10.1016/j.canlet.2013.10.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Purposes: To investigate whether miR-15a inhibits cell proliferation and epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PDAC) via the down-regulation of B cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) expression. Methods and Results: miR-15a and Bmi-1 expressions in normal pancreatic tissue and PDAC tissue were measured. The relationship between miR-15a and Bmi-1 expression was analyzed. We found that miR15a suppressed the expression of Bmi-1 and PDAC cell proliferation; E-cadherin expression was visibly up-regulated after silencing Bmi-1 by transfecting miR-15a into PDAC cell line. Conclusion: miR-15a inhibits cell proliferation and EMT in PDAC via the down-regulation of Bmi-1 expression. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：40 / 46

页数：7

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