Protein tethered lipid bilayer: An alternative mimic of the biological membrane (Mini Review)

被引：12

作者：

Naumann, Renate L. C. ^{[1
]}

Knoll, Wolfgang ^{[1
]}

机构：

[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

来源：

BIOINTERPHASES | 2008年 / 3卷 / 02期

关键词：

biomembranes; lipid bilayers; proteins;

D O I：

10.1116/1.2936939

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An overview is given about results obtained so far with an alternative concept of solid-supported tethered lipid bilayers for the functional incorporation of membrane proteins. The incorporated protein itself acts as the tethering molecule resulting in a versatile system where the protein determines the characteristics of submembraneous space. This architecture is achieved through a metal chelating surface, onto which histidine-tagged (his-tagged) membrane proteins are able to bind in a reversible manner. The tethered bilayer membrane is generated by substitution of protein bound detergent molecules with lipids using in situ dialysis or adsorption. Histidine-tagged cytochrome c oxidase is used as a model protein in this study. However, the system should be applicable to all recombinant membrane proteins bearing a terminal his tag. The system is particularly designed, among other surface-analytical techniques, for a combined application of electrochemical and vibrational spectroscopy measurements.

引用

页码：FA101 / FA107

页数：7

共 31 条

[21] RAPID PURIFICATION OF WILDTYPE AND MUTANT CYTOCHROME-C-OXIDASE FROM RHODOBACTER-SPHAEROIDES BY NI2+-NTA AFFINITY-CHROMATOGRAPHY [J].

MITCHELL, DM ;

GENNIS, RB .

FEBS LETTERS, 1995, 368 (01) :148-150

[22] PHOTOINDUCED ELECTRON-TRANSFER FROM TRIS(2,2'-BIPYRIDYL)RUTHENIUM TO CYTOCHROME-C-OXIDASE [J].

NILSSON, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6497-6501

[23] Cytochrome c oxidase -: structure, function, and physiology of a redox-driven molecular machine [J].

Richter, OMH ;

Ludwig, B .

REVIEWS OF PHYSIOLOGY, BIOCHEMISTRY AND PHARMACOLOGY, VOL 147 2003, 2003, 147 :47-74

[24] Reduction of cytochrome c oxidase by a second electron leads to proton translocation [J].

Ruitenberg, M ;

Kannt, A ;

Bamberg, E ;

Fendler, K ;

Michel, H .

NATURE, 2002, 417 (6884) :99-102

[25] Supported membranes: Scientific and practical applications [J].

Sackmann, E .

SCIENCE, 1996, 271 (5245) :43-48

[26] Supported membranes on soft polymer cushions: fabrication, characterization and applications [J].

Sackmann, E ;

Tanaka, M .

TRENDS IN BIOTECHNOLOGY, 2000, 18 (02) :58-64

[27] Archaea analogue thiolipids for tethered bilayer lipid membranes on ultrasmooth gold surfaces [J].

Schiller, SM ;

Naumann, R ;

Lovejoy, K ;

Kunz, H ;

Knoll, W .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (02) :208-+

[28] SUPPORTED PHOSPHOLIPID-BILAYERS [J].

TAMM, LK ;

MCCONNELL, HM .

BIOPHYSICAL JOURNAL, 1985, 47 (01) :105-113

[29] Polymer-supported membranes as models of the cell surface [J].

Tanaka, M ;

Sackmann, E .

NATURE, 2005, 437 (7059) :656-663

[30] Proton translocation by cytochrome c oxidase [J].

Verkhovsky, MI ;

Jasaitis, A ;

Verkhovskaya, ML ;

Morgan, JE ;

Wikström, M .

NATURE, 1999, 400 (6743) :480-483

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