Site-specific protein modification through CuI-catalyzed 1,2,3-triazole formation and its implementation in protein microarray fabrication

被引：144

作者：

Lin, Po-Chiao ^{[1
]}

Ueng, Shau-Hua

Tseng, Mei-Chun

Ko, Jia-Ling

Huang, Kuo-Ting

Yu, Sheng-Chieh

Adak, AviJit Kumar

Chen, Yu-Ju

Lin, Chun-Cheng

机构：

[1] Natl Tsing Hua Univ, Dept Chem, Hsinchu, Taiwan

[2] Acad Sinica, Taiwan Int Grad Program, Taipei, Taiwan

[3] Acad Sinica, Inst Chem, Taipei, Taiwan

[4] Acad Sinica, Genom Res Ctr, Taipei, Taiwan

[5] Natl Taiwan Normal Univ, Dept Chem, Taipei, Taiwan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 26期

关键词：

copper; dimerization; ligation; nitrogen heterocycles; protein modifications;

D O I：

10.1002/anie.200600756

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Out of site: A protein expression system was combined with CuI-catalyzed 1,2,3-triazole formation to modify a target protein at its C terminus. The immobilized core protein can be modified by diverse small molecules in a site-specific manner (see picture). Protein conjugation with a diazido linker gives a homodimeric protein, and microarrays can be fabricated by site-specific covalent binding to functionalized surfaces. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：4286 / 4290

页数：5

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