Polyelectrolyte-modified short microchannel for cation separation

被引:23
作者
Bai, XX [1 ]
Roussel, C [1 ]
Jensen, H [1 ]
Girault, HH [1 ]
机构
[1] Ecole Polytech Fed Lausanne, Inst Chim Mol & Biol, Lab Electrochim Phys & Analyt, CH-1015 Lausanne, Switzerland
关键词
capillary electrophoresis; cation separation; microchannel; miniaturization; polyelectrolyte; surface modification;
D O I
10.1002/elps.200305771
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Three alkali cations, potassium, sodium, and lithium, have been separated within 15 s in a 1 cm long polymer microchip. The separation microchannel is modified by a polycation, poly(allylammonium chloride), which makes the channel surfaces positively charged leading to a reversed electroosmotic flow (EOF) when compared to bare channels. Due to the decreased apparent mobility of the cations, the separation resolution is improved allowing the use of shorter channels.
引用
收藏
页码:931 / 935
页数:5
相关论文
共 37 条
[11]   Evaluation of coelectroosmotic capillary zone electrophoresis for the rapid analysis of twelve aromatic sulphonate compounds [J].
Cugat, MJ ;
Borrull, F ;
Calull, M .
CHROMATOGRAPHIA, 1999, 49 (5-6) :261-267
[12]  
Dolník V, 2000, ELECTROPHORESIS, V21, P41, DOI 10.1002/(SICI)1522-2683(20000101)21:1<41::AID-ELPS41>3.0.CO
[13]  
2-7
[14]   EFFECT OF THE CONCENTRATION OF 18-CROWN-6 ADDED TO THE ELECTROLYTE UPON THE SEPARATION OF AMMONIUM, ALKALI AND ALKALINE-EARTH CATIONS BY CAPILLARY ELECTROPHORESIS [J].
FRANCOIS, C ;
MORIN, P ;
DREUX, M .
JOURNAL OF CHROMATOGRAPHY A, 1995, 706 (1-2) :535-553
[15]  
GARDENIERS H, 2002, MST NEWS, V4, P39
[16]   Capillaries modified by polyelectrolyte multilayers for electrophoretic separations [J].
Graul, TW ;
Schlenoff, JB .
ANALYTICAL CHEMISTRY, 1999, 71 (18) :4007-4013
[17]   INDIRECT FLUOROMETRIC DETECTION OF CATIONS IN CAPILLARY ZONE ELECTROPHORESIS [J].
GROSS, L ;
YEUNG, ES .
ANALYTICAL CHEMISTRY, 1990, 62 (05) :427-431
[18]   CURRENT-MONITORING METHOD FOR MEASURING THE ELECTROOSMOTIC FLOW-RATE IN CAPILLARY ZONE ELECTROPHORESIS [J].
HUANG, XH ;
GORDON, MJ ;
ZARE, RN .
ANALYTICAL CHEMISTRY, 1988, 60 (17) :1837-1838
[19]   Open-tubular capillary electrochromatography using a polymeric surfactant coating [J].
Kamande, MW ;
Kapnissi, CP ;
Zhu, XF ;
Akbay, C ;
Warner, IM .
ELECTROPHORESIS, 2003, 24 (06) :945-951
[20]  
Kubán P, 2002, ELECTROPHORESIS, V23, P3725, DOI 10.1002/1522-2683(200211)23:21<3725::AID-ELPS3725>3.0.CO