Peptic and tryptic digestion of peptides and proteins monitored by capillary electrophoresis with contactless conductivity detection

被引：33

作者：

Schuchert-Shi, Aiping ^{[1
]}

Hauser, Peter C. ^{[1
]}

机构：

[1] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 387卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

Peptic digestion; Tryptic digestion; Off-line; On-line; EMMA; Capillary electrophoresis; Contactless conductivity detection; AMINO-ACIDS; MEDIATED MICROANALYSIS; MICROCHIP ELECTROPHORESIS; ZONE-ELECTROPHORESIS; FUNDAMENTAL-ASPECTS; MASS-SPECTROMETRY; STRAY CAPACITANCE; SEPARATION; ONLINE; SYSTEM;

D O I：

10.1016/j.ab.2009.01.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The feasibility of monitoring the peptic and tryptic digestion of peptides and proteins with capillary electrophoresis using contactless conductivity detection was investigated. The peptide minigastrin I and the proteins cytochrome c from bovine heart, human serum albumin (HSA), myoglobin, and bovine serum albumin (BSA) were digested off-line with pepsin, and the resulting peptide and amino acid fragments were successfully separated and detected by conductivity measurement. Cytochrome c and myoglobin were also subjected to off-line cleavage with trypsin. On-line digestion using the electrophoretically mediated microanalysis (EMMA) approach was demonstrated with cytochrome c and apomyoglobin using trypsin. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：202 / 207

页数：6

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