Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture

被引：18

作者：

Bettinger, Christopher J. ^{[2
,3
]}

Kulig, Katherine M. ^{[4
]}

Vacanti, Joseph P. ^{[4
]}

Langer, Robert ^{[1
]}

Borenstein, Jeffrey T. ^{[3
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[3] Ctr Biomed Engn, Charles Stark Draper Lab, Cambridge, MA USA

[4] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Boston, MA USA

来源：

TISSUE ENGINEERING PART A | 2009年 / 15卷 / 06期

关键词：

EXTRACELLULAR-MATRIX; GENE-EXPRESSION; BIODEGRADABLE ELASTOMERS; CELL; SCAFFOLDS; ADHESION; BIOMATERIALS; ORGANIZATION; MORPHOLOGY; FEATURES;

D O I：

10.1089/ten.tea.2008.0134

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Synthetic substrates that mimic the properties of extracellular matrix proteins hold significant promise for use in systems designed for tissue engineering applications. In this report, we designed a synthetic polymeric substrate that is intended to mimic chemical, mechanical, and topological characteristics of collagen. We found that elastomeric poly(ester amide) substrates modified with replica-molded nanotopographic features enhanced initial attachment, spreading, and adhesion of primary rat hepatocytes. Further, hepatocytes cultured on nanotopographic substrates also demonstrated reduced albumin secretion and urea synthesis, which is indicative of strongly adherent hepatocytes. These results suggest that these engineered substrates can function as synthetic collagen analogs for in vitro cell culture.

引用

页码：1321 / 1329

页数：9

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