Lipid Modification of Proteins through Sortase-Catalyzed Transpeptidation

被引：119

作者：

Antos, John M. ^{[1
]}

Miller, Gwenn M. ^{[1
]}

Grotenbreg, Gijsbert M. ^{[1
]}

Ploegh, Hidde L. ^{[1
]}

机构：

[1] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 48期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1021/ja806779e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A general chemoenzymatic method for the site-specific attachment of lipids to protein substrates is described. Sortase A is used to append short lipid-modified oligoglycine peptides to the C terminus of protein substrates bearing a five amino acid sortase A recognition sequence (LPETG). We demonstrate the attachment of a range of hydrophobic modifications in excellent yield (60-90%), including a simple step for removing the sortase enzyme postreaction. Lipoproteins prepared using these procedures were subsequently shown to associate with mammalian cells in a lipid tail-dependent fashion and localized to the plasma membrane and endosomes.

引用

收藏

页码：16338 / 16343

页数：6

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