A massively parallel microfluidics platform for storage and ultra high throughput screening

被引：10

作者：

Brenan, CJ ^{[1
]}

Morrison, T ^{[1
]}

Stone, K ^{[1
]}

Heitner, T ^{[1
]}

Katz, A ^{[1
]}

Kanigan, TS ^{[1
]}

Hess, R ^{[1
]}

Kwon, SJ ^{[1
]}

Jung, HC ^{[1
]}

Pan, JG ^{[1
]}

机构：

[1] BioTrove Inc, Cambridge, MA 02139 USA

来源：

BIOMEDICAL NANOTECHNOLOGY ARCHITECTURES AND APPLICATIONS | 2002年 / 4626卷

关键词：

microaray; microfluidics; drug discovery; ultra high throughput screening;

D O I：

10.1117/12.472061

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a novel microarray technology for performing very large numbers (potentially up to one million) of biochemical, chemical and cell-based nanoliter volume synthesis, storage and screening operations in a massively parallel manner. The Living Chip(TM) is an array of precisely machined through-holes retaining nanoliters of fluid by capillary action. Sample loading, washing and recovery are operations that can be performed manually or with simple automation. Mixing between co-registered through-holes is achieved by stacking two or more precision aligned arrays and optical assay read-outs are in parallel with a CCD imaging system. An automated picker system transfers hits into lower density microtiter plates for further analysis. We will present results demonstrating massively parallel implementation of both homogeneous and inhomogeneous fluidic and cell-based assay systems and applications of the chip for drug compound library storage and management.

引用

页码：560 / 569

页数：10

共 12 条

[1] SELF-ORGANIZATION OF ORGANIC LIQUIDS ON PATTERNED SELF-ASSEMBLED MONOLAYERS OF ALKANETHIOLATES ON GOLD [J].