Fabricating large arrays of microwells with arbitrary dimensions and filling them using discontinuous dewetting

被引：274

作者：

Jackman, RJ ^{[1
]}

Duffy, DC ^{[1
]}

Ostuni, E ^{[1
]}

Willmore, ND ^{[1
]}

Whitesides, GM ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 11期

关键词：

D O I：

10.1021/ac971295a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the fabrication of large (up to 45 cm(2)) arrays of microwells, with volumes as small as similar to 3 fL/well and densities as high as 10(7) wells/cm(2). These arrays of microwells are formed by casting an elastomer, poly(dimethylsiloxane) (PDMS), against "masters" prepared by photolithography; arrays of microwells in other polymers can be formed by using a master consisting of posts in PDMS. A straightforward technique, discontinuous dewetting, allows wells to be filled rapidly (typically on the order of 10(4) wells/s) and uniformly with a wide range of liquids. Several rudimentary strategies for addressing microwells are investigated, including electroosmotic pumping and gaseous diffusion.

引用

页码：2280 / 2287

页数：8

共 41 条

[1]

AIZENBERG J, 1997, UNPUB

[2] SELF-ORGANIZATION OF ORGANIC LIQUIDS ON PATTERNED SELF-ASSEMBLED MONOLAYERS OF ALKANETHIOLATES ON GOLD [J].

BIEBUYCK, HA ;

WHITESIDES, GM .

LANGMUIR, 1994, 10 (08) :2790-2793

[3] High-density oligonucleotide arrays [J].

Blanchard, AP ;

Kaiser, RJ ;

Hood, LE .

BIOSENSORS & BIOELECTRONICS, 1996, 11 (6-7) :687-690

[4] ELECTROCHEMICAL MEASUREMENTS IN SUBMICROLITER VOLUMES [J].

BOWYER, WJ ;

CLARK, ME ;

INGRAM, JL .

ANALYTICAL CHEMISTRY, 1992, 64 (04) :459-462

[5] Micromachining sensors for electrochemical measurement in subnanoliter volumes [J].

Bratten, CDT ;

Cobbold, PH ;

Cooper, JM .

ANALYTICAL CHEMISTRY, 1997, 69 (02) :253-258

[6] DIRECT MEASUREMENT OF INTERFACIAL INTERACTIONS BETWEEN SEMISPHERICAL LENSES AND FLAT SHEETS OF POLY(DIMETHYLSILOXANE) AND THEIR CHEMICAL DERIVATIVES [J].

CHAUDHURY, MK ;

WHITESIDES, GM .

LANGMUIR, 1991, 7 (05) :1013-1025

[7] Electrochemical analysis in picoliter microvials [J].

Clark, RA ;

Hietpas, PB ;

Ewing, AG .

ANALYTICAL CHEMISTRY, 1997, 69 (02) :259-263

[8] Capillary flow as the cause of ring stains from dried liquid drops [J].

Deegan, RD ;

Bakajin, O ;

Dupont, TF ;

Huber, G ;

Nagel, SR ;

Witten, TA .

NATURE, 1997, 389 (6653) :827-829

[9] WETTING CHARACTERISTICS OF LIQUID-DROPS AT HETEROGENEOUS SURFACES [J].

DRELICH, J ;

MILLER, JD ;

KUMAR, A ;

WHITESIDES, GM .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1994, 93 :1-13

[10] MICROCOLUMN SAMPLE INJECTION BY SPONTANEOUS FLUID DISPLACEMENT [J].

FISHMAN, HA ;

SCHELLER, RH ;

ZARE, RN .

JOURNAL OF CHROMATOGRAPHY A, 1994, 680 (01) :99-107

← 1 2 3 4 5 →