MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques

被引：103

作者：

Wang, Chong ^{[1
,2
]}

Zheng, Xuexing ^{[2
,3
,4
]}

Gai, Weiwei ^{[2
]}

Zhao, Yongkun ^{[2
,4
]}

Wang, Hualei ^{[2
,4
]}

Wang, Haijun ^{[2
]}

Feng, Na ^{[2
,4
]}

Chi, Hang ^{[2
]}

Qiu, Boning ^{[2
]}

Li, Nan ^{[2
]}

Wang, Tiecheng ^{[2
,4
]}

Gao, Yuwei ^{[2
,4
]}

Yang, Songtao ^{[2
,4
]}

Xia, Xianzhu ^{[2
,4
]}

机构：

[1] Northeast Forestry Univ, Coll Wildlife Resources, Harbin, Peoples R China

[2] Acad Mil Med Sci, Inst Mil Vet, Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun, Peoples R China

[3] Shandong Univ, Sch Publ Hlth, Jinan, Peoples R China

[4] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

来源：

ONCOTARGET | 2017年 / 8卷 / 08期

关键词：

Middle East respiratory syndrome coronavirus; vaccine; virus-like particles; nonhuman primates; immune response; EAST RESPIRATORY SYNDROME; RECEPTOR-BINDING DOMAIN; SYNDROME CORONAVIRUS; NEUTRALIZING ANTIBODIES; SPIKE PROTEIN; VACCINE DEVELOPMENT; MOUSE MODEL; INFECTION; EFFICACY; SYSTEMS;

D O I：

10.18632/oncotarget.8475

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and spread. In this study, we generated MERS-CoV VLPs using the baculovirus expression system. Electron microscopy and immunoelectron microscopy results demonstrate that MERS-CoV VLPs are structurally similar to the native virus. Rhesus macaques inoculated with MERS-CoV VLPs and Alum adjuvant induced virus-neutralizing antibodies titers up to 1: 40 and induced specific IgG antibodies against the receptor binding domain (RBD), with endpoint titers reaching 1:1,280. MERS-CoV VLPs also elicited T-helper 1 cell (Th1)-mediated immunity, as measured by ELISpot. These data demonstrate that MERS-CoV VLPs have excellent immunogenicity in rhesus macaques, and represent a promising vaccine candidate.

引用

页码：12686 / 12694

页数：9

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