Marked enhancement of the antigen-specific immune response by combining plasmid DNA-Based immunization with a Schiff base-forming drug

被引:8
作者
Charo, J
Sundbäck, M
Wasserman, K
Ciupitu, AMT
Mirzai, B
van der Zee, R
Kiessling, R
机构
[1] Karolinska Hosp, CCK, S-17176 Stockholm, Sweden
[2] Karolinska Inst, Microbiol & Tumor Biol Ctr, S-17177 Stockholm, Sweden
[3] Amer Type Culture Collect, Manassas, VA 20110 USA
[4] Univ Utrecht, Dept Immunol, Fac Vet Med, NL-3508 TD Utrecht, Netherlands
关键词
D O I
10.1128/IAI.70.12.6652-6657.2002
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Although plasmid DNA (pDNA)-based immunization has proven efficacy, the level of immune responses that is achieved by this route of vaccination is often lower than that induced by traditional vaccines, especially for primates and humans. We report here a simple and potent method to enhance pDNA-based vaccination by using two different plasmids encoding viral or bacterial antigens. This method is based on coadministration of low concentrations of a recently described immunopotentiating, Schiff base-forming drug called tucaresol which has led to significant augmentation of antigen-specific humoral and cellular immune responses. Our data suggest that enhancement of the immune response with tucaresol might provide a powerful tool for the further development of pDNA-based immunization for humans.
引用
收藏
页码:6652 / 6657
页数:6
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