An improved DNA biosensor built by layer-by-layer covalent attachment of multi-walled carbon nanotubes and gold nanoparticles

被引：60

作者：

Zhang, Yuzhong ^{[1
]}

Ma, Haiyan ^{[1
]}

Zhang, Keying ^{[1
]}

Zhang, Shaojun ^{[1
]}

Wang, Jie ^{[1
]}

机构：

[1] Anhui Normal Univ, Anhui Key Lab Chem Biosensing, Coll Chem & Mat Sci, Wuhan 241000, Peoples R China

来源：

ELECTROCHIMICA ACTA | 2009年 / 54卷 / 08期

关键词：

DNA biosensor; Multi-walled carbon nanotubes; Gold nanoparticles; Layer-by-layer assembly; Covalent attachment; PLATINUM NANOPARTICLES; GLUCOSE-OXIDASE; FILMS; FUNCTIONALIZATION;

D O I：

10.1016/j.electacta.2008.10.052

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

In this work, we synthesized a type of compound, MWCNTs-CONH-(CH2)(2)-SH, via carboxylation, and investigated a thickness-tunable multilayer films DNA biosensor built by layer-by-layer (LBL) covalent attachment of gold nanoparticles (GNPs) and multi-walled carbon nanotubes (MWCNTs) on an Au electrode. Fourier transform infrared (FT-IR) spectra were used to identify the products formed in the step of carboxylation: scanning electron microscopy (SEM) and cyclic voltammetry (CV) were used to study the film assembly process. The hybridization events were monitored through measurement the signal of intercalated doxorubicin by differential pulse voltammetry (DPV), and the oxidation peak currents show a good linear relationship with the logarithm of the concentration of target DNA from 5.0 x 10(-10) to 1.0 x 10(-11) M with a detection limit of 6.2 pM. The improved DNA biosensor has a good stability and reproducibility. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2385 / 2391

页数：7

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