Immobilization, stability and esterification studies of a lipase from a Bacillus sp.

被引：71

作者：

Dosanjh, NS ^{[1
]}

Kaur, J ^{[1
]}

机构：

[1] Panjab Univ, Dept Biotechnol, Chandigarh 160014, India

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2002年 / 36卷 / 01期

关键词：

enzymic reaction; ester synthesis; half-life; thermostability;

D O I：

10.1042/BA20010070

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present paper a comparative account of the immobilization of a Bacillus lipase on different solid supports with different surface properties and their thermostability is presented. Immobilization enhanced the thermostability of lipase. At higher temperatures, lipase immobilized and cross-linked on a hydrophobic surface showed the maximum thermostability. The optimum temperature for immobilized lipase was 9 degreesC higher than for the free enzyme, while the pH optima were the same. The half-life of soluble lipase at 50 degreesC was calculated to be 4.5 h, while immobilized lipase did not lose any activity even after 8 h. The temperature stability (for 1 h) of immobilized enzyme was enhanced from 50 to 60 degreesC in comparison with non-immobilized enzyme. Applications of immobilized lipase for esterification are also presented.

引用

页码：7 / 12

页数：6

共 19 条

[11]

MONSON P, 1983, METHOD ENZYMOL, V137, P584

[12] Purification, characterization and thermostability of lipase from a thermophilic Bacillus sp J33 [J].

Nawani, N ;

Kaur, J .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 2000, 206 (1-2) :91-96

[13] Enzymatic esterification of ethanol by an immobilised Rhizomucor miehei lipase in a perforated rotating disc bioreactor [J].

Oliveira, AC ;

Rosa, MF ;

Aires-Barros, MR ;

Cabral, JMS .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 26 (5-6) :446-450

[14]

Pandey A, 1999, BIOTECHNOL APPL BIOC, V29, P119

[15] Lipase-catalyzed synthesis of tricaprylin in a medium solely composed of substrates. Water production and elimination [J].

Selmi, B ;

Gontier, E ;

Ergan, F ;

Barbotin, JN ;

Thomas, D .

ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (05) :322-325

[16] LIPASE ACTIVITY OF THERMOPHILIC BACTERIA FROM ICELANDIC HOT-SPRINGS [J].

SIGURGISLADOTTIR, S ;

KONRAOSDOTTIR, M ;

JONSSON, A ;

KRISTJANSSON, JK ;

MATTHIASSON, E .

BIOTECHNOLOGY LETTERS, 1993, 15 (04) :361-366

[17] Influence of the organic solvents on the activity in water and the conformation of Candida rugosa lipase: Description of a lipase-activating pretreatment [J].

Torres, C ;

Otero, C .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 19 (08) :594-600

[18] An investigation of crude lipases for hydrolysis, esterification, and transesterification [J].

Wu, XY ;

Jaaskelainen, S ;

Linko, YY .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 19 (03) :226-231

[19] CONTINUOUS HYDROLYSIS OF OLIVE OIL BY IMMOBILIZED LIPASE IN ORGANIC-SOLVENT [J].

YANG, DS ;

RHEE, JS .

BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (06) :748-752

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