Extracellular matrix imitation utilizing nanofibers-embedded biomimetic scaffolds for facilitating cartilage regeneration

被引：55

作者：

Cheng, Gu ^{[1
,2
,3
,4
]}

Dai, Jinhong ^{[1
,2
,3
,4
,5
]}

Dai, Jiawei ^{[1
,2
,3
]}

Wang, Hang ^{[1
,2
,3
]}

Chen, Shuo ^{[1
,2
,3
]}

Liu, Yingheng ^{[1
,2
,3
]}

Liu, Xiayi ^{[1
,2
,3
]}

Li, Xiaoran ^{[6
]}

Zhou, Xue ^{[7
]}

Deng, Hongbing ^{[4
,6
]}

Li, Zhi ^{[1
,2
,3
]}

机构：

[1] Wuhan Univ, Wuhan Univ Stomatol Hosp, State Key Lab Breeding Base Basic Sci Stomatol Hu, Wuhan 430079, Peoples R China

[2] Wuhan Univ, Wuhan Univ Stomatol Hosp, Key Lab Oral Biomed, Minist Educ,Sch & Hosp Stomatol, Wuhan 430079, Peoples R China

[3] Wuhan Univ, Wuhan Univ Stomatol Hosp, Dept Oral & Maxillofacial Trauma & TMJ Surg, Wuhan 430079, Peoples R China

[4] Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resource Chem & Environm Bi, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China

[5] Wenzhou Med Univ, Sch & Hosp Stomatol, Wenzhou 325000, Peoples R China

[6] Donghua Univ, Innovat Ctr Text Sci & Technol, Shanghai 200051, Peoples R China

[7] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Dept Occupat & Environm Hlth, Wuhan 430030, Peoples R China

来源：

CHEMICAL ENGINEERING JOURNAL | 2021年 / 410卷

关键词：

Silk fibroin; Chitin; Nanofibers; Scaffold; Cartilage regeneration;

D O I：

10.1016/j.cej.2020.128379

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

Repair of cartilage legions remains a big challenge for clinicians due to the limit self-regeneration ability of cartilage. A SF-based scaffold that reinforced by the SF-chitin hybrid nanofibers and impregnated with TGF-beta 1 is developed to mimic extracellular matrix and facilitate cartilage regeneration. The composite scaffold with an excellent biocompatibility exhibited the suitable elasticity and hardness after incorporation of the SF-chitin hybrid nanofibers. The attachment and proliferation of chondrocytes was also improved by a rough morphology created by the SF-chitin hybrid nanofibers. As a chondrogenic growth factor, TGF-beta 1 was incorporated into the composite scaffold and modulated the performance of scaffolds by inducing cells homing. In the treatment of rat cartilaginous defects, the SF-chitin hybrid nanofiber-functionalized composite scaffold loaded with TGF-beta 1 remarkably accelerated the cartilage regeneration. Thus, the SF-chitin hybrid nanofiber/TGF-beta 1-functionalized scaffold fabricated in this study promotes the adhesion and proliferation of chondrocytes in vitro, and cartilage regeneration in vivo, thus offering a suitable strategy for the repair of cartilage defects.

引用

页数：14

共 56 条

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