The open-access high-throughput crystallization facility at EMBL Hamburg

被引:62
作者
Mueller-Dieckmann, Jochen [1 ]
机构
[1] DESY, EMBL Hamburg Outstn, D-22603 Hamburg, Germany
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2006年 / 62卷
关键词
D O I
10.1107/S0907444906038121
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Here, the establishment of Europe's largest high-throughput crystallization facility with open access to the general user community is reported. The facility covers every step in the crystallization process from the preparation of crystallization cocktails for initial or customized screens to the setup of hanging-drop vapour-diffusion experiments and their automatic imaging. In its first year of operation, 43 internal and 40 external users submitted over 500 samples for a total of 2985 crystallization plates. An electronic booking system for registration, the selection of experimental parameters (e. g. drop size, sample-to-reservoir ratio) and the reservation of time slots was developed. External users can choose from more than 1000 initial crystallization conditions. By default, experiments are kept for six months and are imaged 15 times during this time period. A remote viewing system is available to inspect experiments via the internet. Over 100 stock solutions are available for users wishing to design customized screens.
引用
收藏
页码:1446 / 1452
页数:7
相关论文
共 12 条
[1]  
Albeck S, 2005, ACTA CRYSTALLOGR D, V61, P1364, DOI 10.1107/S090744905023565
[2]   The Protein Data Bank [J].
Berman, HM ;
Battistuz, T ;
Bhat, TN ;
Bluhm, WF ;
Bourne, PE ;
Burkhardt, K ;
Iype, L ;
Jain, S ;
Fagan, P ;
Marvin, J ;
Padilla, D ;
Ravichandran, V ;
Schneider, B ;
Thanki, N ;
Weissig, H ;
Westbrook, JD ;
Zardecki, C .
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 :899-907
[3]   NEW DEVELOPMENTS OF THE IMPAX SMALL-VOLUME AUTOMATED CRYSTALLIZATION SYSTEM [J].
CHAYEN, NE ;
STEWART, PDS ;
BALDOCK, P .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :456-458
[4]   Efficient protein crystallization [J].
DeLucas, LJ ;
Bray, TL ;
Nagy, L ;
McCombs, D ;
Chernov, N ;
Hamrick, D ;
Cosenza, L ;
Belgovskiy, A ;
Stoops, B ;
Chait, A .
JOURNAL OF STRUCTURAL BIOLOGY, 2003, 142 (01) :188-206
[5]   Vapor diffusion, nucleation rates and the reservoir to crystallization volume ratio [J].
Forsythe, EL ;
Maxwell, DL ;
Pusey, M .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 :1601-1605
[6]   A nanovolume crystallization robot that creates its crystallization screens on-the-fly [J].
Hazes, B ;
Price, L .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2005, 61 :1165-1171
[7]   An integrated approach to structural genomics [J].
Heinemann, U ;
Frevert, J ;
Hofmann, KP ;
Illing, G ;
Maurer, C ;
Oschkinat, H ;
Saenger, W .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2000, 73 (05) :347-362
[8]   A fully integrated protein crystallization platform for small-molecule drug discovery [J].
Hosfield, D ;
Palan, J ;
Hilgers, M ;
Scheibe, D ;
McRee, DE ;
Stevens, RC .
JOURNAL OF STRUCTURAL BIOLOGY, 2003, 142 (01) :207-217
[9]   The high-speed Hydra-Plus-One system for automated high-throughput protein crystallography [J].
Krupka, HI ;
Rupp, B ;
Segelke, BW ;
Lekin, TP ;
Wright, D ;
Wu, HC ;
Todd, P ;
Azarani, A .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 :1523-1526
[10]   A deliberate approach to screening for initial crystallization conditions of biological macromolecules [J].
Luft, JR ;
Collins, RJ ;
Fehrman, NA ;
Lauricella, AM ;
Veatch, CK ;
DeTitta, GT .
JOURNAL OF STRUCTURAL BIOLOGY, 2003, 142 (01) :170-179