A deliberate approach to screening for initial crystallization conditions of biological macromolecules

被引：241

作者：

Luft, JR

Collins, RJ

Fehrman, NA

Lauricella, AM

Veatch, CK

DeTitta, GT

机构：

[1] Hauptman Woodward Inst, Buffalo, NY 14203 USA

[2] SUNY Buffalo, Dept Biol Struct, Buffalo, NY 14203 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2003年 / 142卷 / 01期

关键词：

crystallization; macromolecule; high throughput; microbatch;

D O I：

10.1016/S1047-8477(03)00048-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method to rationally predict crystallization conditions for a previously uncrystallized macromolecule has not yet been developed. One way around this problem is to determine initial crystallization conditions by casting a wide net, surveying a large number of chemical and physical conditions to locate crystallization leads. A facility that executes the rapid survey of crystallization lead conditions is described in detail. Results and guidelines for the initial screening of crystallization conditions, applicable to both manual and robotic setups, are discussed. (C) 2003 Published by Elsevier Science (USA).

引用

页码：170 / 179

页数：10

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