Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection

被引：23

作者：

Pang, Tao

Bai, Changmin

Xu, Yanjuan

Xu, Guowang ^{[1
]}

Yuan, Zhongyi

Su, Yong

Peng, Liming

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China

[2] Chinese Acad Sci, Biochem & Cell Biol Inst, Shanghai Inst Biol Sci, Shanghai, Peoples R China

[3] Chuxiong Cigarette Co, Hongta Grp, Yunnan, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2006年 / 29卷 / 09期

关键词：

solid-phase extraction; liquid chromatography; evaporative light scattering; tobacco leaf; sugars;

D O I：

10.1080/10826070600598993

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A reversed phase liquid chromatography-evaporative light scattering detection, combined with solid phase extraction method was developed for the separation and determination of sugars in tobacco leaf. Separation was performed with a Hypersil NH2 analytical column and the mobile phase 75%:25% acetonitrile-water. In the range of 0.1-4 mg/mL, the method showed good linearity, with the correlation coefficients exceeding 0.99. The limits of detection of xylose, fructose, glucose, sucrose, maltose, and raffinose were 2, 2, 3, 2, 4, and 4 mu g/mL, respectively. The relative standard deviations of peak areas for these sugars were in the range of 2-3%. Recoveries of fructose, glucose, and sucrose were between 96 and 117%. Finally, determination of sugars in tobacco leaf, before and after bio-catalysis, was performed with this method.

引用

页码：1281 / 1289

页数：9

共 22 条

[1]

Simultaneous determination by capillary gas chromatography of organic acids, sugars, and sugar alcohols in plant tissue extracts as their trimethylsilyl derivatives [J].