Determination of theophylline and its metabolites in biological samples by liquid chromatography-mass spectrometry

被引：58

作者：

Kanazawa, H

Atsumi, R

Matsushima, Y

Kizu, J

机构：

[1] Kyoritsu Coll Pharm, Tokyo 1058512, Japan

[2] Univ Tokyo, Dept Pharm, Branch Hosp, Fac Med,Bunkyo Ku, Tokyo 1128688, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 870卷 / 1-2期

关键词：

column switching; theophylline; caffeine;

D O I：

10.1016/S0021-9673(99)00891-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites. We used a column-switching system in combination with atmospheric pressure chemical ionization LC-MS (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. The separation was carried out on a reversed-phase column using methanol-20 mM ammonium acetate as a mobile phase at a flow-rate of 1 ml/min in 30 min. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs. (C) 2000 Elsevier Science BN. All rights reserved.

引用

页码：87 / 96

页数：10

共 11 条

[1] BIOTRANSFORMATION OF CAFFEINE AND THEOPHYLLINE IN MAMMALIAN-CELL LINES GENETICALLY ENGINEERED FOR EXPRESSION OF SINGLE CYTOCHROME-P450 ISOFORMS [J].