Extensive genomic conservation of cattle microsatellite heterozygosity in sheep

被引:51
作者
deGortari, MJ
Freking, BA
Kappes, SM
Leymaster, KA
Crawford, AM
Stone, RT
Beattie, CW
机构
[1] USDA ARS, US MEAT ANIM RES CTR, CLAY CTR, NE 68933 USA
[2] UNIV OTAGO, DEPT BIOCHEM, AGRES MOL BIOL UNIT, DUNEDIN, NEW ZEALAND
关键词
heterozygosity; microsatellite; ovine;
D O I
10.1111/j.1365-2052.1997.00153.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
We report the evaluation of 1036 bovine microsatellite primer pairs for their suitability as linkage markers in sheep. Approximately 58% (605/1036) of bovine primer pairs amplified a locus in sheep. Sixty-seven per cent (409/605) of amplified loci were detected as polymorphic. Marker heterozygosity, allele number and range of allele sizes were significantly lower in sheep than cattle sampled in this study. However, median fragment size was similar, These data suggest that high-resolution comparative linkage maps between closely related species can be constructed relatively efficiently.
引用
收藏
页码:274 / 290
页数:17
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