Medium chain length polyhydroxyalkanoate (mcl-PHA) production from volatile fatty acids derived from the anaerobic digestion of grass

被引:55
作者
Cerrone, Federico [1 ,2 ,3 ]
Choudhari, Santosh K. [1 ,4 ]
Davis, Reeta [1 ,2 ,3 ]
Cysneiros, Denise [1 ,5 ]
O'Flaherty, Vincent [1 ,5 ]
Duane, Gearoid [6 ]
Casey, Eoin [6 ]
Guzik, Maciej W. [2 ,3 ]
Kenny, Shane T. [2 ,3 ]
Babu, Ramesh P. [1 ,4 ,7 ]
O'Connor, Kevin [1 ,2 ,3 ]
机构
[1] Natl Univ Ireland, Technol Ctr Biorefining & Bioenergy, Galway, Ireland
[2] Univ Coll Dublin, UCD Conway Inst, Sch Biomol & Biomed Sci, Belfield Dublin 4, Ireland
[3] Univ Coll Dublin, Earth Inst, Belfield Dublin 4, Ireland
[4] Univ Dublin Trinity Coll, Ctr Res Adapt Nanostruct & Nanodevices, Dublin 2, Ireland
[5] Natl Univ Ireland, Sch Nat Sci & Environm Change, Dept Microbiol, Galway, Ireland
[6] Univ Coll Dublin, Sch Biochem & Bioproc Engn, Belfield Dublin 4, Ireland
[7] Univ Dublin Trinity Coll, Sch Phys, Dublin 2, Ireland
关键词
mcl-Polyhydroxyalkanoates; Grass biomass; Biorefining; Anaerobic digestion; VFAs; PSEUDOMONAS-PUTIDA CA-3; MICROBIAL COMMUNITY; METABOLIC ANALYSIS; WASTE-WATER; BIOSYNTHESIS; HYDROLYSIS; DEGRADATION; NITROGEN; CONSTRUCTION; ACCUMULATION;
D O I
10.1007/s00253-013-5323-x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A two step biological process for the conversion of grass biomass to the biodegradable polymer medium chain length polyhydroxyalkanoate (mcl-PHA) was achieved through the use of anaerobic and aerobic microbial processes. Anaerobic digestion (mixed culture) of ensiled grass was achieved with a recirculated leach bed bioreactor resulting in the production of a leachate, containing 15.3 g/l of volatile fatty acids (VFAs) ranging from acetic to valeric acid with butyric acid predominating (12.8 g/l). The VFA mixture was concentrated to 732.5 g/l with a 93.3 % yield of butyric acid (643.9 g/l). Three individual Pseudomonas putida strains, KT2440, CA-3 and GO16 (single pure cultures), differed in their ability to grow and accumulate PHA from VFAs. P. putida CA-3 achieved the highest biomass and PHA on average with individual fatty acids, exhibited the greatest tolerance to higher concentrations of butyric acid (up to 40 mM) compared to the other strains and exhibited a maximum growth rate (mu(MAX) = 0.45 h(-1)). Based on these observations P. putida CA-3 was chosen as the test strain with the concentrated VFA mixture derived from the AD leachate. P. putida CA-3 achieved 1.56 g of biomass/l and accumulated 39 % of the cell dry weight as PHA (nitrogen limitation) in shake flasks. The PHA was composed predominantly of 3-hydroxydecanoic acid (> 65 mol%).
引用
收藏
页码:611 / 620
页数:10
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