Easy to optimize: Dynamic combinatorial libraries of metal-dye complexes as flexible sensors for tripeptides

被引：47

作者：

Buryak, Andrey ^{[1
]}

Severin, Kay ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Sci & Ingn Chim, Lausanne, Switzerland

来源：

JOURNAL OF COMBINATORIAL CHEMISTRY | 2006年 / 8卷 / 04期

关键词：

D O I：

10.1021/cc060028s

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A dynamic combinatorial library (DCL) of dye complexes of Cu2+ and Ni2+ was used to sense the sequence isomeric tripeptides His-Gly-Gly, Gly-His-Gly, and Gly-Gly-His. The DCL sensor was obtained by mixing buffered aqueous solution of three commercially available dyes together with CuCl2 and NiCl2. The addition of the peptide analytes resulted in characteristic changes in the UV-vis spectrum of the mixture, which allowed the identification of the peptide. Due to the modular nature of the sensor, it was possible to optimize the sensor by variation of the amounts and ratio of its constituent building blocks. The composition of the best sensor was found to vary substantially, depending on the sensing problem to be addressed. Whereas a Cu2+/Ni2+ ratio of 1:3 gave the best differentiation for Gly-His-Gly and Gly-Gly-His, a sensor containing exclusively Cu2+ was found to provide the best discrimination between His-Gly-Gly and Gly-Gly-His.

引用

页码：540 / 543

页数：4

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