Monolithic columns with mixed modes of reversed-phase and anion-exchange stationary phase for capillary electrochromatography

被引：41

作者：

Fu, HJ ^{[1
]}

Xie, CH ^{[1
]}

Xiao, H ^{[1
]}

Dong, J ^{[1
]}

Hu, JW ^{[1
]}

Zou, HF ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1044卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

monolithic columns; mixed-mode separations; stationary phases; electrochromatography; anilines; alkylbenzenes; polynuclear aromatic hydrocarbons; caffeine; barbitals; benzoic acids; terephthalic acid;

D O I：

10.1016/j.chroma.2004.04.076

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A capillary electrochromatography (CEC) monolithic column with mixed modes of reversed-phase and anion-exchange stationary phases was prepared by in situ polymerization of 2-(methacryloxy)ethyltrimethylammonium methyl sulfate (MEAMS) and ethylene dimethacrylate (EDMA) in a binary porogenic solvent consisting 1-propanol and 1,4-butanediol. The ammonium groups on the surface of the stationary phase generate an electroosmotic flow (EOF) from cathode to anode, and serve as a strong anion-exchange stationary phase at the same time. The EOF of the stationary phase can be determined by the amount of MEAMS monomer in reaction mixtures during the polymerization. The monolithic stationary phases exhibited reversed-phase chromatographic behavior toward neutral solutes. For charged solutes, hydrophobic as well as electrostatic interaction/repulsion with the monoliths was observed. Separations of aromatic compounds and basic compounds on the prepared column were performed under the mode of CEC. Peak tailing of basic compounds was avoided and the efficient separation of aromatic acids was achieved using neutral mobile phase due to the same direction of EOF and electrophorestic mobility of negatively charged solutes. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：237 / 244

页数：8

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