Complement consumption by poly(ethylene glycol) in different conformations chemically coupled to poly(isobutyl 2-cyanoacrylate) nanoparticles

被引:139
作者
Peracchia, MT [1 ]
Vauthier, C [1 ]
Passirani, C [1 ]
Couvreur, P [1 ]
Labarre, D [1 ]
机构
[1] UNIV PARIS SUD,CNRS,URA 1218,F-92296 CHATENAY MALABR,FRANCE
关键词
nanoparticles; complement consumption; opsonization; poly(ethylene glycol); poly(alkyleyanoacrylate);
D O I
10.1016/S0024-3205(97)00539-0
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
There is an increasing interest to develop injectable drug polymeric carriers not recognizable by the body as foreign particles and eliminated very quickly from the bloodstream. A polyethylene glycol (PEG)-coating onto injectable particles showed to reduce either protein adsorption and complement consumption, as a function of the PEG density. In this work we compared the complement rejecting ability of PEG in different conformations coupled to polyisobutylcyanoacrylate (PIBCA) nanoparticles, through the analysis of the residual hemolytic capacity of the human serum after contact with the particles. Nanoparticles were formed by chemical coupling of PEG during emulsion/polymerization of isobutylcyanoacrylate (IBCA). Nanoparticles characterization included an investigation of their surface properties, such as hydrophilicity and conformational mobility of the PEG chains grafted on the nanoparticles surface, and PEG total content. The polymerization kinetics of IBCA in presence of PEG or MePEG were also studied. Complement consumption was observed to be very sensitive to the number of particles in contact with human serum, as well as to the PEG conformation, suggesting PEG configuration could affect the particle exposed surface.
引用
收藏
页码:749 / 761
页数:13
相关论文
共 31 条
[21]  
2-N
[22]  
PERACCHIA MT, 1996, P INT S CONTR REL BI, V23, P395
[23]  
PETRAK K, 1993, PHARMACEUTICAL PARTI, P275
[24]   ROLE OF COMPLEMENTS-C3 AND COMPLEMENTS-C5 IN THE PHAGOCYTOSIS OF LIPOSOMES BY HUMAN NEUTROPHILS [J].
SCIESZKA, JF ;
MAGGIORA, LL ;
WRIGHT, SD ;
CHO, MJ .
PHARMACEUTICAL RESEARCH, 1991, 8 (01) :65-69
[25]   A METHOD FOR THE ESTIMATION OF POLYETHYLENE-GLYCOL IN PLASMA-PROTEIN FRACTIONS [J].
SIMS, GEC ;
SNAPE, TJ .
ANALYTICAL BIOCHEMISTRY, 1980, 107 (01) :60-63
[26]   SURFACE MODIFICATION OF POLY(LACTIDE-CO-GLYCOLIDE) NANOSPHERES BY BIODEGRADABLE POLY(LACTIDE)-POLY(ETHYLENE GLYCOL) COPOLYMERS [J].
STOLNIK, S ;
DUNN, SE ;
GARNETT, MC ;
DAVIES, MC ;
COOMBES, AGA ;
TAYLOR, DC ;
IRVING, MP ;
PURKISS, SC ;
TADROS, TF ;
DAVIS, SS ;
ILLUM, L .
PHARMACEUTICAL RESEARCH, 1994, 11 (12) :1800-1808
[27]   SURFACE MODIFICATION OF NANOPARTICLES BY PEO PPO BLOCK-COPOLYMERS TO MINIMIZE INTERACTIONS WITH BLOOD COMPONENTS AND PROLONG BLOOD-CIRCULATION IN RATS [J].
TAN, JS ;
BUTTERFIELD, DE ;
VOYCHECK, CL ;
CALDWELL, KD ;
LI, JT .
BIOMATERIALS, 1993, 14 (11) :823-833
[28]   TARGETED DELIVERY OF DIAGNOSTIC AGENTS BY SURFACE-MODIFIED LIPOSOMES [J].
TORCHILIN, VP ;
TRUBETSKOY, VS ;
MILSHTEYN, AM ;
CANILLO, J ;
WOLF, GL ;
PAPISOV, MI ;
BOGDANOV, AA ;
NARULA, J ;
KHAW, BA ;
OMELYANENKO, VG .
JOURNAL OF CONTROLLED RELEASE, 1994, 28 (1-3) :45-58
[29]   CORRELATION OF THE SURFACE HYDROPHOBICITY OF C-14 POLY (METHYL-METHACRYLATE) NANOPARTICLES TO THEIR BODY DISTRIBUTION [J].
TROSTER, SD ;
WALLIS, KH ;
MULLER, RH ;
KREUTER, J .
JOURNAL OF CONTROLLED RELEASE, 1992, 20 (03) :247-260
[30]   Effect of PEO surface density on long-circulating PLA-PEO nanoparticles which are very low complement activators [J].
Vittaz, M ;
Bazile, D ;
Spenlehauer, G ;
Verrecchia, T ;
Veillard, M ;
Puisieux, F ;
Labarre, D .
BIOMATERIALS, 1996, 17 (16) :1575-1581