Quantitative stability of DNA after extended storage of clinical specimens as determined by real-time PCR

被引：128

作者：

Jerome, KR

Huang, ML

Wald, A

Selke, S

Corey, L

机构：

[1] Fred Hutchinson Canc Res Ctr, Program Infect Dis, Seattle, WA 98109 USA

[2] Univ Washington, Dept Lab Med, Seattle, WA 98195 USA

[3] Univ Washington, Dept Med, Seattle, WA 98195 USA

[4] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA

[5] Childrens Hosp & Reg Med Ctr, Virol Sect, Seattle, WA 98105 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 07期

关键词：

D O I：

10.1128/JCM.40.7.2609-2611.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Viral DNA stored for extended periods can be amplified by PCR. However, it is unknown whether stored specimens give accurate quantitative results by newer real-time PCR techniques. We therefore compared herpes simplex virus DNA levels in specimens before and after 16 months of storage. The levels of viral DNA remained stable whether the DNA was stored as purified DNA or unextracted DNA in a whole specimen.

引用

页码：2609 / 2611

页数：3

共 8 条

[1] STORAGE-CONDITIONS OF BLOOD-SAMPLES AND PRIMER SELECTION AFFECT THE YIELD OF CDNA POLYMERASE CHAIN-REACTION PRODUCTS OF HEPATITIS-C VIRUS [J].