Visualization of Dynamics of Single Endogenous mRNA Labeled in Live Mouse

被引：221

作者：

Park, Hye Yoon ^{[1
,2
]}

Lim, Hyungsik ^{[3
,4
]}

Yoon, Young J. ^{[1
]}

Follenzi, Antonia ^{[5
,6
]}

Nwokafor, Chiso ^{[1
,3
,4
,7
]}

Lopez-Jones, Melissa ^{[1
]}

Meng, Xiuhua ^{[1
]}

Singer, Robert H. ^{[1
,2
,8
,9
]}

机构：

[1] Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Gruss Lipper Biophoton Ctr, Bronx, NY 10461 USA

[3] CUNY Hunter Coll, Dept Phys & Astron, New York, NY 10065 USA

[4] CUNY, Grad Ctr, New York, NY 10065 USA

[5] Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10461 USA

[6] Univ Piemonte Orientale, Dept Hlth Sci, Novara, Italy

[7] CUNY Hunter Coll, Dept Biol Sci, New York, NY 10065 USA

[8] Albert Einstein Coll Med, Dominick P Purpura Dept Neurosci, Bronx, NY 10461 USA

[9] Howard Hughes Med Inst, Ashburn, VA 20147 USA

来源：

SCIENCE | 2014年 / 343卷 / 6169期

关键词：

LOCALIZATION; GRANULES; STIMULATION; PROTEIN; GROWTH;

D O I：

10.1126/science.1239200

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The transcription and transport of messenger RNA (mRNA) are critical steps in regulating the spatial and temporal components of gene expression, but it has not been possible to observe the dynamics of endogenous mRNA in primary mammalian tissues. We have developed a transgenic mouse in which all beta-actin mRNA is fluorescently labeled. We found that beta-actin mRNA in primary fibroblasts localizes predominantly by diffusion and trapping as single mRNAs. In cultured neurons and acute brain slices, we found that multiple beta-actin mRNAs can assemble together, travel by active transport, and disassemble upon depolarization by potassium chloride. Imaging of brain slices revealed immediate early induction of beta-actin transcription after depolarization. Studying endogenous mRNA in live mouse tissues provides insight into its dynamic regulation within the context of the cellular and tissue microenvironment.

引用

页码：422 / 424

页数：3

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