Multiplex PCR for diagnosis of enteric infections associated with diarrheagenic Escherichia coli

被引：100

作者：

Vidal, R

Vidal, M

Lagos, R

Levine, M

Prado, V

机构：

[1] Univ Chile, Programa Microbiol, Fac Med, Inst Ciencias Biomed, Santiago, Chile

[2] Univ Maryland, Ctr Vaccine Dev, Baltimore, MD 21201 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 04期

关键词：

D O I：

10.1128/JCM.42.4.1787-1789.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multiplex PCR for detection of three categories of diarrheagenic Escherichia coli was developed. With this method, enterohemorrhagic E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli were identified in fecal samples from patients with hemorrhagic collitis, watery diarrhea, or hemolytic-uremic syndrome and from food-borne outbreaks.

引用

收藏

页码：1787 / 1789

页数：3

相关论文

共 10 条

[1] SIMULTANEOUS IDENTIFICATION OF STRAINS OF ESCHERICHIA-COLI SEROTYPE O157-H7 AND THEIR SHIGA-LIKE TOXIN TYPE BY MISMATCH AMPLIFICATION MUTATION ASSAY-MULTIPLEX PCR [J].

CEBULA, TA ;

PAYNE, WL ;

FENG, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :248-250

[2] Secretion of extracellular proteins by enterohemorrhagic Escherichia coli via a putative type III secretion system [J].

Jarvis, KG ;

Kaper, JB .

INFECTION AND IMMUNITY, 1996, 64 (11) :4826-4829

[3] A GENETIC-LOCUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI NECESSARY FOR THE PRODUCTION OF ATTACHING AND EFFACING LESIONS ON TISSUE-CULTURE CELLS [J].

JERSE, AE ;

YU, J ;

TALL, BD ;

KAPER, JB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) :7839-7843

[4] EPIDEMIOLOGIC STUDIES OF ESCHERICHIA-COLI DIARRHEAL INFECTIONS IN A LOW SOCIOECONOMIC LEVEL PERIURBAN COMMUNITY IN SANTIAGO, CHILE [J].

LEVINE, MM ;

FERRECCIO, C ;

PRADO, V ;

CAYAZZO, M ;

ABREGO, P ;

MARTINEZ, J ;

MAGGI, L ;

BALDINI, MM ;

MARTIN, W ;

MANEVAL, D ;

KAY, B ;

GUERS, L ;

LIOR, H ;

WASSERMAN, SS ;

NATARO, JP .

AMERICAN JOURNAL OF EPIDEMIOLOGY, 1993, 138 (10) :849-869

[5] ESCHERICHIA-COLI THAT CAUSE DIARRHEA - ENTEROTOXIGENIC, ENTEROPATHOGENIC, ENTEROINVASIVE, ENTEROHEMORRHAGIC, AND ENTEROADHERENT [J].

LEVINE, MM .

JOURNAL OF INFECTIOUS DISEASES, 1987, 155 (03) :377-389

[6]

Nataro JP, 1998, CLIN MICROBIOL REV, V11, P403

[7]

Prado V, 2002, REV MED CHILE, V130, P495

[8]

SOTOMAYOR V, 2000, VIGIA, V3, P16

[9] THE PATHOGENIC MECHANISMS OF SHIGA TOXIN AND THE SHIGA-LIKE TOXINS [J].

TESH, VL ;

OBRIEN, AD .

MOLECULAR MICROBIOLOGY, 1991, 5 (08) :1817-1822

[10] Virulence of enterohemorrhagic Escherichia coli:: role of molecular crosstalk [J].

Tesh, VL .

TRENDS IN MICROBIOLOGY, 1998, 6 (06) :228-233