Development of a highly sensitive chemiluminescence flow-injection analysis sensor for phosphate-ion detection using maltose phosphorylase

被引:29
作者
Nakamura, H
Hasegawa, M
Nomura, Y
Arikawa, Y
Matsukawa, R
Ikebukuro, K
Karube, I
机构
[1] Univ Tokyo, Adv Sci & Technol Res Ctr, Meguro Ku, Tokyo 1538904, Japan
[2] Japan Womens Univ, Fac Sci, Dept Chem & Biol Sci, Mejiro Ku, Tokyo 1120015, Japan
关键词
phosphate sensor; chemiluminescence; Arthromyces ramosus peroxidase; N-hydroxysuccinimide beads; flow-injection analysis;
D O I
10.1016/S0168-1656(99)00150-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A chemiluminescence flow-injection analysis biosensor has been constructed for phosphate-ion detection. This system is coenzymeless and employs a maltose phosphorylase, mutarotase, and glucose-oxidase (MP-MUT-GOD) reaction system combined with an Arthromyces ramosus peroxidase-luminol reaction system. The system consists of a column packed with MP-MUT-GOD immobilized on N-hydroxysuccinimide beads, a mixing joint for the chemiluminescence reaction, and a photomultiplier. The response provided by this system was linear, with a wide range between 10 nM and 30 mu M phosphate ion, and a measuring time of 3 min per sample. Under the optimal condition, the sensor was able to detect 1.0 mu M phosphate ion for at least 2 weeks. For a practical application, the determination of phosphate ion in river water was examined using ethylenediaminetetraacetic acid, and the results were estimated by comparing with the molybdenum-blue method. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:127 / 133
页数:7
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