Characterization of zebrafish larval inflammatory macrophages

被引：112

作者：

Mathias, Jonathan R. ^{[1
]}

Dodd, M. Ernest ^{[1
]}

Walters, Kevin B. ^{[1
]}

Yoo, Sa Kan ^{[1
]}

Ranheim, Erik A. ^{[2
]}

Huttenlocher, Anna ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA

[2] Univ Wisconsin, Sch Med & Publ Hlth, Dept Pathol & Lab Med, Madison, WI 53792 USA

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2009年 / 33卷 / 11期

关键词：

Zebrafish; Inflammation; Macrophage; Neutrophil; Phagocytosis; IN-VIVO; TRANSGENIC ZEBRAFISH; DEFINITIVE HEMATOPOIESIS; GENE-EXPRESSION; DANIO-RERIO; NEUTROPHILS; MIGRATION; ENGRAFTMENT; CHEMOTAXIS; PHAGOCYTES;

D O I：

10.1016/j.dci.2009.07.003

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Zebrafish have emerged as a powerful model system to study leukocyte recruitment and inflammation. Here we characterize the morphology and function of inflammatory macrophages in zebrafish larvae. These macrophages can be distinguished from neutrophils by immunolabeling of L-Plastin without MPO co-expression and by an elongated morphology. Live imaging of transgenic zMPO:GFP larvae demonstrate that GFP(lo) macrophages migrate to wounds by extension of thin pseudopods and carry out phagocytosis of tissue debris, and FACS analysis of leukocyte markers indicates expression of CSF1R in these macrophages. These findings identify distinct functional and morphological characteristics of inflammatory macrophages in zebrafish larvae. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1212 / 1217

页数：6

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