Following dimethyl sulfoxide skin optical clearing dynamics with quantitative nonlinear multimodal microscopy

被引：61

作者：

Zimmerley, Maxwell ^{[1
]}

McClure, R. Anthony ^{[2
]}

Choi, Bernard ^{[3
,4
]}

Potma, Eric Olaf ^{[1
,4
]}

机构：

[1] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Dept Neurobiol, Irvine, CA 92697 USA

[3] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA

[4] Univ Calif Irvine, Beckman Laser Inst, Irvine, CA 92697 USA

来源：

APPLIED OPTICS | 2009年 / 48卷 / 10期

关键词：

RAMAN SCATTERING MICROSCOPY; 2ND-HARMONIC GENERATION; HYPEROSMOTIC AGENTS; COHERENCE TOMOGRAPHY; COLLAGEN-I; TISSUE; CONTRAST; FLUORESCENCE; SPECTROSCOPY; ENHANCEMENT;

D O I：

10.1364/AO.48.000D79

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Second-harmonic generation (SHG) imaging is combined with coherent anti-Stokes Raman scattering (CARS) microscopy to follow the process of optical clearing in human skin ex vivo using dimethyl sulfoxide (DMSO) as the optical clearing agent. SHG imaging revealed that DMSO introduces morphological changes to the collagen I matrix. By carefully measuring the dynamic tissue attenuation of the coherent nonlinear signal, using CARS reference signals during the clearing process, it is found that DMSO reduces the overall SHG response from dermal collagen. Evidence is provided for a role of DMSO in compromising the structure of collagen fibers, associated with a reduction of the tissue's scattering properties. (C) 2009 Optical Society of America

引用

页码：D79 / D87

页数：9

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