Thickness dependence of optical second harmonic generation in collagen fibrils

被引:28
作者
Chu, Shi-Wei
Tai, Shih-Peng
Chan, Ming-Che
Sun, Chi-Kuang
Hsiao, I-Ching
Lin, Chi-Hung
Chen, Yung-Chih
Lin, Bai-Ling
机构
[1] Natl Taiwan Univ, Dept Phys, Taipei 10617, Taiwan
[2] Natl Taiwan Univ, Grad Inst Photon & Optoelect, Dept Elect Engn, Taipei 10617, Taiwan
[3] Acad Sinica, Res Ctr Appl Sci, Taipei 115, Taiwan
[4] Natl Yang Ming Univ, Inst Microbiol & Immunol, Taipei 11221, Taiwan
[5] Baker Heart Res Inst, Melbourne, Vic 3004, Australia
[6] Natl Taiwan Univ, Inst Plant Biol, Taipei 10617, Taiwan
来源
OPTICS EXPRESS | 2007年 / 15卷 / 19期
关键词
IN-VIVO; MICROSCOPY; FLUORESCENCE; TISSUES; IMAGES; CELLS;
D O I
10.1364/OE.15.012005
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Simultaneous backward and forward second harmonic generations from isolated type-I collagen matrix are observed. Optical interference behaviors of these nonlinear optical signals are studied with accurately determined fibril thickness by an atomic force microscope. The nonlinear emission directions are strongly dependent on the coherent interaction within and between collagen fibrils. A linear relationship is obtained to estimate collagen fibril thickness with nanometer precision noninvasively by evaluating the forward/backward second harmonic generation ratio. (c) 2007 Optical Society of America.
引用
收藏
页码:12005 / 12010
页数:6
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