Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells

被引：1129

作者：

Saito, Yoshimasa ^{[1
]}

Liang, Gangning ^{[1
]}

Egger, Gerda ^{[1
]}

Friedman, Jeffrey M. ^{[1
]}

Chuang, Jody C. ^{[1
]}

Coetzee, Gerhard A. ^{[1
]}

Jones, Peter A. ^{[1
]}

机构：

[1] Univ So Calif, Norris Comprehens Canc Ctr, Dept Urol Biochem & Mol Biol, Los Angeles, CA 90089 USA

来源：

CANCER CELL | 2006年 / 9卷 / 06期

关键词：

D O I：

10.1016/j.ccr.2006.04.020

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Expression profiling of T24 cells revealed that 17 out of 313 human miRNAs were upregulated more than 3-fold by simultaneous treatment with the chromatin-modifying drugs 5-aza-2'-deoxycytidine and 4-phenylbutyric acid. One of these, miR-127, is embedded in a CpG island and is highly induced from its own promoter after treatment. miR-127 is usually expressed as part of a miRNA cluster in normal cells but not in cancer cells, suggesting that it is subject to epigenetic silencing. In addition, the proto-oncogene BCL6, a potential target of miR-127, was translationally downregulated after treatment. These results suggest that DNA demethylation and histone deacetylase inhibition can activate expression of miRNAs that may act as tumor suppressors.

引用

页码：435 / 443

页数：9

共 29 条

[1] Clustering and conservation patterns of human microRNAs [J].

Altuvia, Y ;

Landgraf, P ;

Lithwick, G ;

Elefant, N ;

Pfeffer, S ;

Aravin, A ;

Brownstein, MJ ;

Tuschl, T ;

Margalit, H .

NUCLEIC ACIDS RESEARCH, 2005, 33 (08) :2697-2706

[2] The functions of animal microRNAs [J].

Ambros, V .

NATURE, 2004, 431 (7006) :350-355

[3] Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs [J].

Cai, XZ ;

Hagedorn, CH ;

Cullen, BR .

RNA, 2004, 10 (12) :1957-1966

[4] Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia [J].

Calin, GA ;

Dumitru, CD ;

Shimizu, M ;

Bichi, R ;

Zupo, S ;

Noch, E ;

Aldler, H ;

Rattan, S ;

Keating, M ;

Rai, K ;

Rassenti, L ;

Kipps, T ;

Negrini, M ;

Bullrich, F ;

Croce, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) :15524-15529

[5] Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer [J].

Cameron, EE ;

Bachman, KE ;

Myöhänen, S ;

Herman, JG ;

Baylin, SB .

NATURE GENETICS, 1999, 21 (01) :103-107

[6] Real-time quantification of microRNAs by stem-loop RT-PCR [J].

Chen, CF ;

Ridzon, DA ;

Broomer, AJ ;

Zhou, ZH ;

Lee, DH ;

Nguyen, JT ;

Barbisin, M ;

Xu, NL ;

Mahuvakar, VR ;

Andersen, MR ;

Lao, KQ ;

Livak, KJ ;

Guegler, KJ .

NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9

[7] miR-15 and miR-16 induce apoptosis by targeting BCL2 [J].

Cimmino, A ;

Calin, GA ;

Fabbri, M ;

Iorio, MV ;

Ferracin, M ;

Shimizu, M ;

Wojcik, SE ;

Aqeilan, RI ;

Zupo, S ;

Dono, M ;

Rassenti, L ;

Alder, H ;

Volinia, S ;

Liu, CG ;

Kipps, TJ ;

Negrini, M ;

Croce, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (39) :13944-13949

[8] Demethylation of a hypermethylated P15/INK4B gene in patients with myelodysplastic syndrome by 5-Aza-2′-deoxycytidine (decitabine) treatment [J].

Daskalakis, M ;

Nguyen, TT ;

Nguyen, C ;

Guldberg, P ;

Köhler, G ;

Wijermans, P ;

Jones, PA ;

Lübbert, M .

BLOOD, 2002, 100 (08) :2957-2964

[9] RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus [J].

Davis, E ;

Caiment, F ;

Tordoir, X ;

Cavaillé, J ;

Ferguson-Smith, A ;

Cockett, N ;

Georges, M ;

Charlier, C .

CURRENT BIOLOGY, 2005, 15 (08) :743-749

[10] Epigenetics in human disease and prospects for epigenetic therapy [J].

Egger, G ;

Liang, GN ;

Aparicio, A ;

Jones, PA .

NATURE, 2004, 429 (6990) :457-463

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