A paper-based calorimetric microfluidics platform for bio-chemical sensing

被引:47
作者
Davaji, Benyamin [1 ]
Lee, Chung Hoon [1 ]
机构
[1] Marquette Univ, Nanoscale Devices Lab, Milwaukee, WI 53233 USA
关键词
Micro-calorimetry; Thermal bio-sensor; Label-free detection; Paper-based sensor; TIME;
D O I
10.1016/j.bios.2014.03.022
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In this report, a paper-based micro-calorimetric biochemical detection method is presented. Calorimetric detection of biochemical reactions is demonstrated as an extension of current colorimetric and electrochemical detection mechanisms of paper-based biochemical analytical systems. Reaction and/or binding temperature of glucose/glucose oxidase, DNA/hydrogen peroxide, and biotin/streptavidin, are measured by the paper-based micro-calorimeter. Commercially available glucose calibration samples of 0.05, 0.15 and 0.3% wt/vol concentration are used for comparing the device performance with a commercially available glucose meter (electrochemical detection). The calorimetric glucose detection demonstrates a measurement error less than 2%. The calorimetric detection results of DNA concentrations from 0.9 to 7.3 mg/mL and temperature changes in biotin and streptavidin reaction are presented to demonstrate the feasibility of integrating the calorimetric detection method with paper based microfluidic devices. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:120 / 126
页数:7
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