Preparation of Glycopolymer-Substituted Gold Nanoparticles and Their Molecular Recognition

被引:63
作者
Toyoshima, Masayuki [1 ]
Miura, Yoshiko [1 ]
机构
[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi, Ishikawa 9231292, Japan
关键词
glycopolymer; molecular recognition; nanoparticles; reversible addition fragmentation chain transfer (RAFT); QUANTITATIVE COLORIMETRIC DETECTION; LIVING RADICAL POLYMERIZATION; SELF-ASSEMBLED MONOLAYERS; RAFT POLYMERIZATION; SURFACE; FACILE; LECTIN; GLYCONANOPARTICLES; AGGREGATION; CLUSTERS;
D O I
10.1002/pola.23250
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Glycopolymer-substituted gold nanoparticles were prepared via living radical polymerization with a reversible addition-fragmentation chain transfer (RAFT) reagent. Polyacrylamide derivatives with alpha-mannose (alpha-Man) and N-acetyl-beta-glucosamine (beta-GlcNAc) were synthesized and hydrogenated to obtain thiol-terminated polymer. The thiol-terminated glycopolymers were mixed with gold nanoparticles to yield the polymer substituted gold nanoparticles with various diameters, which aggregated on addition of saccharide-recognition proteins (lectins). The aggregation properties were analyzed using transmission electron microscopy and UV spectra. Molecular recognition was studied with E. coli, which induced aggregation of the nanoparticles at the cell periphery. (C) 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 1412-1421, 2009
引用
收藏
页码:1412 / 1421
页数:10
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