Molecular characterization of Brucella strains isolated from marine mammals

被引:105
作者
Bricker, BJ
Ewalt, DR
MacMillan, AP
Foster, G
Brew, S
机构
[1] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA
[2] Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, USDA, Ames, IA USA
[3] FAO WHO Ctr Brucellosis Reference & Res, OIE Brucellosis Reference Lab, Cent Vet Lab, Weybridge, Surrey, England
关键词
D O I
10.1128/JCM.38.3.1258-1262.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as Brucella species by conventional phenotypic analysis. This study found the 16S rRNA gene from one representative isolate was identical to the homologous sequences of Brucella abortus, B. melitensis, B. canis, and B. suis. IS711-based DNA fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical Brucella species. In general, fingerprint patterns grouped by host species. The data suggest that the marine mammal isolates are distinct types of Brucella and not one of the classical species or biovars invading new host species. In keeping with historical precedent, the designation of several new Brucella species may be appropriate.
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页码:1258 / 1262
页数:5
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