Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation

被引：15

作者：

Horsey, I ^{[1
]}

Krishnan-Ghosh, Y ^{[1
]}

Balasubramanian, S ^{[1
]}

机构：

[1] Univ Cambridge, Chem Labs, Cambridge CB2 1EW, England

来源：

CHEMICAL COMMUNICATIONS | 2002年 / 17期

关键词：

D O I：

10.1039/b206054j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Metal chelating iminodiacetic acid moieties were appended to abutting ends of two 9-mer oligonucleotides designed to hybridize contiguously on an 18-mer target. Chelation of 1 equivalent of Gd3+ cooperatively increases affinities of the oligonucleotides to the single stranded DNA target as evidenced by a 15 degreesC increase in T-m of the complex.

引用

页码：1950 / 1951

页数：2

共 23 条

[1] COOPERATIVE OLIGONUCLEOTIDE-DIRECTED TRIPLE-HELIX FORMATION AT ADJACENT DNA SITES [J].

COLOCCI, N ;

DISTEFANO, MD ;

DERVAN, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (11) :4468-4473

[2] Regioselective effect of zwitterionic DNA substitutions on DNA alkylation: Evidence for a strong side chain orientational preference [J].

Dande, P ;

Liang, GN ;

Chen, FX ;

Roberts, C ;

Nelson, MG ;

Hashimoto, H ;

Switzer, C ;

Gold, B .

BIOCHEMISTRY, 1997, 36 (20) :6024-6032

[3] Novel phosphoramidite monomer for the site-selective incorporation of a diastereochemically pure phosphoramidate to oligonucleotide [J].

Endo, M ;

Komiyama, M .

JOURNAL OF ORGANIC CHEMISTRY, 1996, 61 (06) :1994-2000

[4] MODULATION OF OLIGONUCLEOTIDE DUPLEX AND TRIPLEX STABILITY VIA HYDROPHOBIC INTERACTIONS [J].

GRYAZNOV, SM ;

LLOYD, DH .

NUCLEIC ACIDS RESEARCH, 1993, 21 (25) :5909-5915

[5] Synthesis and hybridization analysis of a small library of peptide-oligonucleotide conjugates [J].

Harrison, JG ;

Balasubramanian, S .

NUCLEIC ACIDS RESEARCH, 1998, 26 (13) :3136-3145

[6]

Hill TerrellL., 1985, Cooperativity Theory in Biochemistry: Steady-state and Equilibrium Systems

[7] Metal ion-directed cooperative triple helix formation of glutamic acid-oligonucleotide conjugate [J].

Ihara, T ;

Takeda, Y ;

Jyo, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (08) :1772-1773

[8] ON THE ATTRIBUTION AND ADDITIVITY OF BINDING-ENERGIES [J].

JENCKS, WP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (07) :4046-4050

[9] DESIGN, BIOCHEMICAL, BIOPHYSICAL AND BIOLOGICAL PROPERTIES OF COOPERATIVE ANTISENSE OLIGONUCLEOTIDES [J].

KANDIMALLA, ER ;

MANNING, A ;

LATHAN, C ;

BYRN, RA ;

AGRAWAL, S .

NUCLEIC ACIDS RESEARCH, 1995, 23 (17) :3578-3584

[10] Preorganization of DNA: Design principles for improving nucleic acid recognition by synthetic oligonucleotides [J].

Kool, ET .

CHEMICAL REVIEWS, 1997, 97 (05) :1473-1487

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