Adult mouse cortical cell taxonomy revealed by single cell transcriptomics

被引：1103

作者：

Tasic, Bosiljka ^{[1
]}

Menon, Vilas ^{[1
]}

Thuc Nghi Nguyen ^{[1
]}

Kim, Tae Kyung ^{[1
]}

Jarsky, Tim ^{[1
]}

Yao, Zizhen ^{[1
]}

Levi, Boaz ^{[1
]}

Gray, Lucas T. ^{[1
]}

Sorensen, Staci A. ^{[1
]}

Dolbeare, Tim ^{[1
]}

Bertagnolli, Darren ^{[1
]}

Goldy, Jeff ^{[1
]}

Shapovalova, Nadiya ^{[1
]}

Parry, Sheana ^{[1
]}

Lee, Changkyu ^{[1
]}

Smith, Kimberly ^{[1
]}

Bernard, Amy ^{[1
]}

Madisen, Linda ^{[1
]}

Sunkin, Susan M. ^{[1
]}

Hawrylycz, Michael ^{[1
]}

Koch, Christof ^{[1
]}

Zeng, Hongkui ^{[1
]}

机构：

[1] Allen Inst Brain Sci, Seattle, WA USA

来源：

NATURE NEUROSCIENCE | 2016年 / 19卷 / 02期

基金：

美国国家卫生研究院;

关键词：

RNA-SEQ; LAYER; 6; NEUROGLIAFORM CELLS; GABAERGIC NEURONS; GENE-EXPRESSION; TRANSGENIC MICE; VISUAL-CORTEX; DRIVER LINES; INTERNEURONS; CONNECTIONS;

D O I：

10.1038/nn.4216

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Nervous systems are composed of various cell types, but the extent of cell type diversity is poorly understood. We constructed a cellular taxonomy of one cortical region, primary visual cortex, in adult mice on the basis of single-cell RNA sequencing. We identified 49 transcriptomic cell types, including 23 GABAergic, 19 glutamatergic and 7 non-neuronal types. We also analyzed cell type-specific mRNA processing and characterized genetic access to these transcriptomic types by many transgenic Cre lines. Finally, we found that some of our transcriptomic cell types displayed specific and differential electrophysiological and axon projection properties, thereby confirming that the single-cell transcriptomic signatures can be associated with specific cellular properties.

引用

收藏

页码：335 / +

页数：18

相关论文

共 81 条

[51] Electrophysiological differences between neurogliaform cells from monkey and rat prefrontal cortex [J].

Povysheva, N. V. ;

Zaitsev, A. V. ;

Kroner, S. ;

Krimer, O. A. ;

Rotaru, D. C. ;

Gonzalez-Burgos, G. ;

Lewis, D. A. ;

Krimer, L. S. .

JOURNAL OF NEUROPHYSIOLOGY, 2007, 97 (02) :1030-1039

[52] Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells [J].

Ramskold, Daniel ;

Luo, Shujun ;

Wang, Yu-Chieh ;

Li, Robin ;

Deng, Qiaolin ;

Faridani, Omid R. ;

Daniels, Gregory A. ;

Khrebtukova, Irina ;

Loring, Jeanne F. ;

Laurent, Louise C. ;

Schroth, Gary P. ;

Sandberg, Rickard .

NATURE BIOTECHNOLOGY, 2012, 30 (08) :777-782

[53] High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells [J].

Raymond, Christopher S. ;

Soriano, Philippe .

PLOS ONE, 2007, 2 (01)

[54] limma powers differential expression analyses for RNA-sequencing and microarray studies [J].

Ritchie, Matthew E. ;

Phipson, Belinda ;

Wu, Di ;

Hu, Yifang ;

Law, Charity W. ;

Shi, Wei ;

Smyth, Gordon K. .

NUCLEIC ACIDS RESEARCH, 2015, 43 (07) :e47

[55] Melanocortin-4 Receptors Expressed by Cholinergic Neurons Regulate Energy Balance and Glucose Homeostasis [J].

Rossi, Jari ;

Balthasar, Nina ;

Olson, David ;

Scott, Michael ;

Berglund, Eric ;

Lee, Charlotte E. ;

Choi, Michelle J. ;

Lauzon, Danielle ;

Lowell, Bradford B. ;

Elmquist, Joel K. .

CELL METABOLISM, 2011, 13 (02) :195-204

[56] Cortical fast-spiking parvalbumin interneurons enwrapped in the perineuronal net express the metallopeptidases Adamts8, Adamts15 and Neprilysin [J].

Rossier, J. ;

Bernard, A. ;

Cabungcal, J-H ;

Perrenoud, Q. ;

Savoye, A. ;

Gallopin, T. ;

Hawrylycz, M. ;

Cuenod, M. ;

Do, K. ;

Urban, A. ;

Lein, Ed S. .

MOLECULAR PSYCHIATRY, 2015, 20 (02) :154-161

[57] Three Groups of Interneurons Account for Nearly 100% of Neocortical GABAergic Neurons [J].

Rudy, Bernardo ;

Fishell, Gordon ;

Lee, SooHyun ;

Hjerling-Leffler, Jens .

DEVELOPMENTAL NEUROBIOLOGY, 2011, 71 (01) :45-61

[58]

Sando R, 2013, NAT METHODS, V10, P1085, DOI [10.1038/NMETH.2640, 10.1038/nmeth.2640]

[59] NOS-positive local circuit neurons are exclusively axo-dendritic cells both in the neo- and archicortex of the rat brain [J].

Seress, L ;

Abrahám, H ;

Hajnal, A ;

Lin, H ;

Totterdell, S .

BRAIN RESEARCH, 2005, 1056 (02) :183-190

[60] Single-cell RNA-seq reveals dynamic paracrine control of cellular variation [J].

Shalek, Alex K. ;

Satija, Rahul ;

Shuga, Joe ;

Trombetta, John J. ;

Gennert, Dave ;

Lu, Diana ;

Chen, Peilin ;

Gertner, Rona S. ;

Gaublomme, Jellert T. ;

Yosef, Nir ;

Schwartz, Schraga ;

Fowler, Brian ;

Weaver, Suzanne ;

Wang, Jing ;

Wang, Xiaohui ;

Ding, Ruihua ;

Raychowdhury, Raktima ;

Friedman, Nir ;

Hacohen, Nir ;

Park, Hongkun ;

May, Andrew P. ;

Regev, Aviv .

NATURE, 2014, 510 (7505) :363-+

← 1 2 3 4 5 6 7 8 9 →