PCL-gelatin composite nanofibers electrospun using diluted acetic acid-ethyl acetate solvent system for stem cell-based bone tissue engineering

被引:116
作者
Binulal, N. S. [1 ,2 ]
Natarajan, Amrita [1 ,2 ]
Menon, Deepthy [1 ,2 ]
Bhaskaran, V. K. [2 ,3 ]
Mony, Ullas [1 ,2 ]
Nair, Shantikumar V. [1 ,2 ]
机构
[1] Amrita Vishwa Vidyapeetham Univ, Amrita Inst Med Sci, Amrita Ctr Nanosci & Mol Med, Kochi, Kerala, India
[2] Amrita Vishwa Vidyapeetham Univ, Res Ctr, Kochi, Kerala, India
[3] Amrita Vishwa Vidyapeetham Univ, Amrita Inst Med Sci, Dept Orthoped, Kochi, Kerala, India
关键词
gelatin; PCL; electrospinning; nanofibers; tissue engineering; mesenchymal stem cells; composite scaffolds; degradation; POLYMERIC NANOFIBERS; IN-VITRO; SCAFFOLDS; DIFFERENTIATION; DELIVERY; ATTACHMENT; GROWTH; FILMS;
D O I
10.1080/09205063.2013.859872
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Composite nanofibrous scaffolds with various poly(epsilon-caprolactone) (PCL)/gelatin ratios (90:10, 80:20, 70:30, 60:40, 50:50wt.%) were successfully electrospun using diluted acetic and ethyl acetate mixture. The effects of this solvent system on the solution properties of the composites and its electrospinning properties were investigated. Viscosity and conductivity of the solutions, with the addition of gelatin, allowed for the electrospinning of uniform nanofibers with increasing hydrophilicity and degradation. Composite nanofibers containing 30 and 40wt.% gelatin showed an optimum combination of hydrophilicity and degradability and also maintained the structural integrity of the scaffold. Human mesenchymal stem cells (hMSCs) showed favorable interaction with and proliferation on, the composite scaffolds. hMSC proliferation was highest in the 30 and 40wt.% gelatin containing composites. Our experimental data suggested that PCL-gelatin composite nanofibers containing 30-40wt.% of gelatin and electrospun in diluted acetic acid-ethyl acetate mixture produced nanofiber scaffolds with optimum hydrophilicity, degradability, and bio-functionality for stem cell-based bone tissue engineering.
引用
收藏
页码:325 / 340
页数:16
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