Targeted genomic rearrangements using CRISPR/Cas technology

被引：234

作者：

Choi, Peter S. ^{[1
,2
]}

Meyerson, Matthew ^{[1
,2
,3
]}

机构：

[1] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02215 USA

[2] Broad Inst MIT & Harvard, Canc Program, Cambridge, MA 02142 USA

[3] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

关键词：

LUNG-CANCER; GENE FUSIONS; ZINC-FINGER; HUMAN-CELLS; CAS9; TRANSLOCATIONS; SPECIFICITY; RET; ADENOCARCINOMA; IDENTIFICATION;

D O I：

10.1038/ncomms4728

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.

引用

页数：6

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