Fluorescence Logic-Signal-Based Multiplex Detection of Nucleases with the Assembly of a Cationic Conjugated Polymer and Branched DNA

被引：95

作者：

Feng, Xuli ^{[1
]}

Duan, Xinrui ^{[1
]}

Liu, Libing ^{[1
]}

Feng, Fude ^{[1
]}

Wang, Shu ^{[1
]}

Li, Yuliang ^{[1
]}

Zhu, Daoben ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100190, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 29期

基金：

中国国家自然科学基金;

关键词：

biosensors; conjugated polymers; DNA cleavage; FRET; nucleases; CLEAVAGE; ASSAYS; COMPLEXES; KINETICS; PROBE;

D O I：

10.1002/anie.200901555

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An energy-transfer cascade is generated from a cationic conjugated polymer (PFP) and negatively charged, Y-shaped DNA labeled with three dyes at its termini (fluorescein (Fl), Tex Red, and Cy5). Multistep fluorescence resonance energy transfer regulates the fluorescence intensities of PFP and the dyes. Different types of logic gates can be operated by observing the emission wavelengths of different dyes with multiplex nucleases as inputs. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5316 / 5321

页数：6

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