Fluorescence Logic-Signal-Based Multiplex Detection of Nucleases with the Assembly of a Cationic Conjugated Polymer and Branched DNA

被引：95

作者：

Feng, Xuli ^{[1
]}

Duan, Xinrui ^{[1
]}

Liu, Libing ^{[1
]}

Feng, Fude ^{[1
]}

Wang, Shu ^{[1
]}

Li, Yuliang ^{[1
]}

Zhu, Daoben ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100190, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 29期

基金：

中国国家自然科学基金;

关键词：

biosensors; conjugated polymers; DNA cleavage; FRET; nucleases; CLEAVAGE; ASSAYS; COMPLEXES; KINETICS; PROBE;

D O I：

10.1002/anie.200901555

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An energy-transfer cascade is generated from a cationic conjugated polymer (PFP) and negatively charged, Y-shaped DNA labeled with three dyes at its termini (fluorescein (Fl), Tex Red, and Cy5). Multistep fluorescence resonance energy transfer regulates the fluorescence intensities of PFP and the dyes. Different types of logic gates can be operated by observing the emission wavelengths of different dyes with multiplex nucleases as inputs. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5316 / 5321

页数：6

共 57 条

[31] Signal-amplifying conjugated Polymer-DNA hybrid chips
Lee, Kangwon
Rouillard, Jean-Marie
Pham, Trinh
Gulari, Erdogan
Kim, Jinsang
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (25) : 4667 - 4670
[32] Lee SP, 1997, METHOD ENZYMOL, V278, P343
[33] A sensitive colorimetric and fluorescent probe based on a polythiophene derivative for the detection of ATP
Li, C
Numata, M
Takeuchi, M
Shinkai, S
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (39) : 6371 - 6374
[34] In vitro selection and characterization of a highly efficient Zn(II)-dependent RNA-cleaving deoxyribozyme
Li, J
Zheng, WC
Kwon, AH
Lu, Y
[J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (02) : 481 - 488
[35] Unique structure and photoluminescence of Au/CdTe nanostructure materials
Li, J
Li, D
Hong, X
Wang, L
Zhao, K
Li, JH
Bai, YB
Li, TJ
[J]. CHEMICAL COMMUNICATIONS, 2004, (08) : 982 - 983
[36] Multiplexed detection of pathogen DNA with DNA-based fluorescence nanobarcodes
Li, YG
Cu, YTH
Luo, D
[J]. NATURE BIOTECHNOLOGY, 2005, 23 (07) : 885 - 889
[37] Controlled assembly of dendrimer-like DNA
Li, YG
Tseng, YD
Kwon, SY
D'Espaux, L
Bunch, JS
Mceuen, PL
Luo, D
[J]. NATURE MATERIALS, 2004, 3 (01) : 38 - 42
[38] Linn S.M., 1993, NUCLEASES
[39] Optimization of the molecular orbital energies of conjugated polymers for optical amplification of fluorescent sensors
Liu, B
Bazan, GC
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (04) : 1188 - 1196
[40] Homogeneous fluorescence-based DNA detection with water-soluble conjugated polymers
Liu, B
Bazan, GC
[J]. CHEMISTRY OF MATERIALS, 2004, 16 (23) : 4467 - 4476

← 1 2 3 4 5 6 →