Investigating antibody-antigen binding with atomic force microscopy

被引：39

作者：

Ouerghi, O

Touhami, A

Othmane, A ^{[1
]}

Ben Ouada, H

Martelet, C

Fretigny, C

Jaffrezic-Renault, N

机构：

[1] Fac Med Monastir, Biophys Lab, Monastir, Tunisia

[2] Fac Sci Monastir, Lab Phys Interfaces, Monastir, Tunisia

[3] Ecole Cent Lyon, UMR 5621, Lab Ingn & Fonctionnalisat Surfaces, F-69131 Ecully, France

[4] ESPCI, Phys Quant Lab, F-75005 Paris 05, France

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2002年 / 84卷 / 2-3期

关键词：

atomic force microscopy; IgG; IgG moieties; immune complex; biosensors;

D O I：

10.1016/S0925-4005(02)00020-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Adhesive interactions between antigen-antibody play a central role in immune system operation. The objective of this paper is to detect immune complexes without any label. by using atomic force microscopy (AFM). We have proceeded by imaging antibody (anti-rabbit IgG) or anti-rabbit IgG moieties adsorbed onto mica surface, before and after incubation of two kinds of antigens: a specific (rabbit IgG) and a nonpecific one (sheep IgG). The analysis using the height histograms reveals many interesting features. We propose a general framework for interpreting height histogram measurements, which enable the discrimination between specific and non-specific complexes. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：167 / 175

页数：9

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